IN-VITRO STUDY ON THE HEMOLYTIC ACTIVITY OF DIFFERENT EXTRACTS OF INDIAN MEDICINAL PLANT CROTON BONPLANDIANUM WITH PHYTOCHEMICAL ESTIMATION: A NEW ERA IN DRUG DEVELOPMENT
Abstract
In this study different extracts of the leaves of Croton bonplandianum were screened for the haemolytic activity towards human erythrocytes. The haemolytic activity was performed by modified spectroscopic method at four different concentrations (300, 150, 75, 25 μg/ml). The haemolytic activity of the different extracts of Croton bonplandianum was found in the following order Ethyl acetate extract > Chloroform extract > Benzene extract. However, all the extracts alone and in combination with each other exhibited very low haemolytic activity. E. ganitrus did not exhibit any haemolytic activity at any dilution. Hence, they can be considered as safe to human erythrocytes.
Keywords: Hemolytic activity, Croton bonplandianum, Erythrocytes
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References
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19. Mathur P. Kapil A. Das B, Dhawan B. Prevalence of ESBL producing gram negative bacteria in a tertiary care hospital. Indian J. Med. Res., 2002; 115:153-157.
20. Sharma S. Bhat G.K. and Shenoy S. Virulence factors and drug resistance in Escherichia coli isolated from extraintestinal infections. Indian J. Med. Microbiol., 2007; 25:369-373.
21. Bahashwan S.A. and El Shafey H.M. Antimicrobial resistance patterns of Proteus isolates from clinical specimens. Eur. Sci. J., 2013; 9:188-202.
2. Saeed S, Tariq P. Antibacterial activities of Mentha piperita, Pisum sativum and Momordica charantia. Pakistan Journal of Botany. 2005; 37:997.
3. Lam S, Chan H, LeRiche JC, Chan-Yeung M, Salari H. Release of leukotrienes in patients with bronchial asthma. Journal of allergy and clinical immunology. 1988; 81:711-7.
4. Grant EN, Lyttle CS, Weiss KB. The relation of socioeconomic factors and racial/ethnic differences in US asthma mortality. American journal of public health. 2000; 90:1923.
5. Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, et al. Contribution of IL-17–producing γδ T cells to the efficacy of anticancer chemotherapy. The Journal of experimental medicine. 2011; 208:491-503.
6. Poonkothai M, Saravanan M. Antibacterial activity of Aegle marmelos against leaf, bark and fruit extracts. Ancient science of life. 2008; 27:15.
7. Davis RJ. MAPKs: new JNK expands the group. Trends in biochemical sciences. 1994; 19:470-3.
8. Dick DM, Foroud T, Flury L, Bowman ES, Miller MJ, Rau NL, et al. Genomewide linkage analyses of bipolar disorder: a new sample of 250 pedigrees from the National Institute of Mental Health Genetics Initiative. The American Journal of Human Genetics. 2003; 73:107-14.
9. Burt S. Essential oils: their antimicrobial properties and potential application in foods-A review. International Journal of Food Microbiology. 2004, 94:223-253.
10. Pauli A, Amicbase. Essential Oils Supplementary Information: Review Science, 90513 Zirndorf, Further Str. 13.
11. Buchbauer G, Jirovetz L. Aromatherapy use of fragrances and essential oils as medicaments. Flav. Fragr. J. 1994, 9: 217 – 222.
12. Hussain A I, Anwar F, Sherazi S T H, Przybylski R. Chemical composition. Antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry. 2008; 108:986-995.
13. Mathur P. Kapil A. Das B., Dhawan B. Prevalence of ESBL producing gram negative bacteria in a tertiary care hospital. Indian J. Med. Res., 2002; 115:153-157.
14. Sharma S. Bhat G.K. and Shenoy S. Virulence factors and drug resistance in Escherichia coli isolated from extraintestinal infections. Indian J. Med. Microbiol., 2007; 25:369-373
15. Bahashwan S.A., El Shafey H.M. Antimicrobial resistance patterns of Proteus isolates from clinical specimens. Eur. Sci. J., 2013; 9:188-202.
16. Parekh, J. and Chanda, S. Antibacterial activity of aqueous and alcoholic extracts of 34 Indian medicinal plants.
17. Buchbauer G, Jirovetz L. Aromatherapy use of fragrances and essential oils as medicaments. Flav. Fragr. J. 1994, 9: 217 – 222.
18. Hussain A I, Anwar F, Sherazi S T H, Przybylski R. Chemical composition. Antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry. 2008, 108; 986-995.
19. Mathur P. Kapil A. Das B, Dhawan B. Prevalence of ESBL producing gram negative bacteria in a tertiary care hospital. Indian J. Med. Res., 2002; 115:153-157.
20. Sharma S. Bhat G.K. and Shenoy S. Virulence factors and drug resistance in Escherichia coli isolated from extraintestinal infections. Indian J. Med. Microbiol., 2007; 25:369-373.
21. Bahashwan S.A. and El Shafey H.M. Antimicrobial resistance patterns of Proteus isolates from clinical specimens. Eur. Sci. J., 2013; 9:188-202.
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How to Cite
Ghosh, T., Biswas, M., Chatterjee, S., & Roy, P. (2018). IN-VITRO STUDY ON THE HEMOLYTIC ACTIVITY OF DIFFERENT EXTRACTS OF INDIAN MEDICINAL PLANT CROTON BONPLANDIANUM WITH PHYTOCHEMICAL ESTIMATION: A NEW ERA IN DRUG DEVELOPMENT. Journal of Drug Delivery and Therapeutics, 8(4), 155-160. https://doi.org/10.22270/jddt.v8i4.1747
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