EVALUATION OF CYTOPROTECTIVE EFFICACY OF ARISAEMA LESCHNAULTII BLUME AGAINST CYCLOPHOSPHAMIDE INDUCED HEPATOTOXICITY IN TUMOR BEARING MICE

  • Yogendra Mavai Department of Pharmacology, Jiwaji University, Gwalior, M.P., India
  • Suman Jain Department of Pharmacology, Jiwaji University, Gwalior, M.P., India

Abstract

Cyclophosphamide (CP) is a widely used antineoplastic drug, it is used for the treatment of several malignancies. However, upon treatment, it induces severe toxicity due to its oxidative stress capability  In this study we evaluate the cytoprtective efficacy of Arisaema leschnaultii blume (AL) against cyclophosphamide induced hepatotoxicity in tumor bearing mice due to its antioxidant property. Female Swiss albino mice were used. Group I-Naive control, Group II-DAL bearing, Group III-DAL + CP, Group IV-  DAL + AL + CP,  Group V- DAL + AL + CP, Group VI-DAL + Amifostine + CP. CP induced hepatic damage as indicated by significant elevation (P < 0.05) in aspartate aminotransferase, organ weight, and evidence by the histological study. CP also induced hepatic oxidative stress as indicated by significant elevation (P < 0.05) in malondialdehyde content, hydrogen peroxide (H2 O2 ) generation, nitrite level, and the level of glutathione (GSH) peroxidase crashed in the CP treated group. AL enhanced the antioxidant defense system as indicated by elevation in GSH level, catalase activity, and GSH‑S‑transferase activity and by inhibiting MDA.

Keywords: hepatotoxicity, glutathione, antioxidants, cytoprotective etc.

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Author Biographies

Yogendra Mavai, Department of Pharmacology, Jiwaji University, Gwalior, M.P., India

Department of Pharmacology, Jiwaji University, Gwalior, M.P., India

Suman Jain, Department of Pharmacology, Jiwaji University, Gwalior, M.P., India

Department of Pharmacology, Jiwaji University, Gwalior, M.P., India

References

1. Lawson M, Vasilaras A, De Vries A, Mactaggart P, Nicol D. Urological implications of cyclophosphamide and ifosfamide. Scand J Urol Nephrol 2008; 42:309 17.
2. Fraiser LH, Kanekal S, Kehrer JP. Cyclophosphamide toxicity. Characterising and avoiding the problem. Drugs 1991; 42:781 95.
3. King PD, Perry MC. Hepatotoxicity of chemotherapy. Oncologist 2001; 6:162 76.
4. Ludeman SM. The chemistry of the metabolites of cyclophosphamide. Curr Pharm Des 1999; 5:627 43.
5. KernJC,KehrerJP.Acrolein inducedcelldeath:Acaspase infuenced decision between apoptosis and oncosis/necrosis. Chem Biol Interact 2002; 139:79 95.
6. Angulo I, Jiménez Díaz MB, García Bustos JF, Gargallo D, de las Heras FG, Muñoz Fernández MA, et al. Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production. Cell Immunol 2002; 218:46 58.
7. Zarei M, Shivanandappa T. Amelioration of cyclophosphamide induced hepatotoxicity by the root extract of Decalepis hamiltonii in mice. Food Chem Toxicol 2013; 57:179 84.
8. Adams JD Jr, Klaidman LK. Acrolein induced oxygen radical formation. Free Radic Biol Med 1993;15:187 93
9. Kouloulias, V.E.; Kouvaris, J.R. Cytoprotective Efficacy of Amifostine against Radiation- Induced Rectal Toxicity: Objective and Subjective Grading Scales for Radiomucositis. Molecules 2008, 13, 892-903.
10. Links M, Lewis C. 1999. Chemoprotectants: a review of their clinical pharmacology and therapeutic efficacy of drugs 1999;57:293-308.
11. Kumar P, Vijayraghwan R, Kulkarni AS, Pathak U, Raza SK, Jaiswal DK. In vivo protection by amifostinen and DRDE-07 against sulphur mustard toxicity. Hum Exp Toxicol 2002; 21:371-76.
12. Pandey N, Barwe D, P nrajapati, Dubey BK, Antioxidant activity of Ethanolic Extract of Arisaema leschenaultii Blume Tuber., research gate
13. Beutler E, Baluda Mc. Methemoglobin reduction. Studies of the interaction between cell populations and of the role of methylene blue. Blood. 1963 Sep; 22:323-33.
14. Ohkawa H, Ohishi N, Yagi K.,Assay for lipid peroxides in animal tissues bthiobarbituric acid reaction. Anal Biochem. 1979 Jun; 95(2):351-8.
15. Sedlak J, Lindsay RHE stimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968 Oct 24; 25(1):192-205.
16. Motchink AP, Frei, Ames NB. Measurment of antioxidants in human plasma. Protein thiols, in oxygen radicals in biological systems. Methods in enzymology, part D, California, Academic press 1994; 234:273-4.
17. Habig WH, Pabst MJ,jakoby WB. Glutathione S transferases. The first enzymatic step in mercapturic acid formation. J Biol chem 1974; 249:7130-39.
18. Harvey JW, Beutler E. Binding of heme by glutathions S-transferases. A possible role of erythrocyte enzyme. Blood 1982; 60:1227-30.
19. D’souza D, Subhas BG, Shetty SR, and Balan P, Estimation of serum malondialdehyde in potentially malignant disorders and post-antioxidant treated patients: A biochemical studyContemp Clin Dent. 2012 Oct-Dec; 3(4): 448–451.
20. Christine J. Weydert and Joseph J. Cullen measurement of superoxide dismutase, catalase, and glutathione peroxidase IN cultured cells and tissue nat Protoc 2010; 5(1): 51–66.
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How to Cite
Mavai, Y., & Jain, S. (2018). EVALUATION OF CYTOPROTECTIVE EFFICACY OF ARISAEMA LESCHNAULTII BLUME AGAINST CYCLOPHOSPHAMIDE INDUCED HEPATOTOXICITY IN TUMOR BEARING MICE. Journal of Drug Delivery and Therapeutics, 8(4), 134-141. https://doi.org/10.22270/jddt.v8i4.1738