PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ASSESSMENT OF HERBAL EXTRACTS
Aim: The present study was aimed to evaluate and compare the antioxidant potential of the methanolic extracts Plumbago zeylanica Linn (PZME), Datura stramonium (DSME) and Argemone mexicana Linn (AMME) with its polyherbal methanol extract (PHME) composition.
Material and method: All the extracts were screened for the presence of various phytochemical by known and standard methods. Qualitative estimation of some potential phytoconstituents like flavonoids and polyphynolics was characterized by TLC (thin layer chromatography) and FTIR (Fourier Transform Infrared Spectroscopy). The quantification of the Rutin (flavonoid) and Gallic acid (polyphenol) were carried out using UV-visible spectrophotometry. The antioxidant activity of methanolic plant extracts and their combinations were assessed by DPPH (1, 1-diphenyl-2-picryl-hydrazil) method. Ascorbic acid was used as a standard and the antioxidant potential were evaluated at 2, 4, 6 and 8 mg/ml concentration.
Results: The phytochemical characterization of PZME, DSME and AMME revealed the presence of tannins, alkaloids, phenols and flavonoids.The polyherbal methanol extract (PHME) was found to exhibit potent antioxidant activity with maximum free radical scavenging activity (FRSA) of 82.79% as compared to standard ascorbic acid (92.92%) at a concentration of 8 mg/ml and was found to be significantly greater than individual methanol plant extracts.
Conclusion: The study revealed a potent synergistic antioxidant effect of methanol extracts of Plumbago zeylanica Linn, Datura stramonium Linn and Argemone mexicana Linn which can be successfully used to mitigate oxidative stress associated complications and other regenerative therapies.
Keywords: Plumbago zeylanica Linn, Datura stramonium Linn, Argemone mexicana Linn, Antioxidant, DPPH (1, 1-diphenyl-2-picryl-hydrazil), FTIR, Rutin, Gallic acid.
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