REACTIVE OXYGEN SPECIES AS POSSIBLE MEDIATOR OF ANTIBACTERIAL ACTIVITY OF PARKIA JAVANICA, AGAINST BACTERIAL SPECIES PREDOMINANTLY FOUND IN CHRONIC WOUND.

  • Samir Kumar Sil Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • Susmita Saha Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • Parimal Karmakar Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.

Abstract

The crude methanol extract of Parkia javanica was screened for antibacterial activity. against bacterial species predominantly found in chronic wound, by serial dilution technique. Growth kinetics study was performed and percentage of ROS production was measured by NBT reduction assay. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained with a range of IC100 5-40 mg/ml in case of standard bacterial strains. The lag phase of all extract treated bacteria is extended compared to untreated cells. The normalized % of ROS is increased in presence of crude extract. This study suggests that the crude methanol extract of Parkia javanica possesses promising antimicrobial substances which are having activity against Standard ATCC bacterial species and ROS induced DNA damage could be the possible mediator of its antimicrobial activity.

Keywords: Parkia javanica, antibacterial activity, standard ATCC bacterial strains, growth curve, ROS, DNA damage


Downloads

Download data is not yet available.

Author Biographies

Samir Kumar Sil, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Susmita Saha, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Parimal Karmakar, Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.

Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.

References

1. Sheipouri D, Braidy N, Guillemin GJ. Kynurenine pathway in skin cells: Implications for UV-induced skin damage. Int J Tryptophan Res 2012; 5: 15-25.
2. Chaitanya SK, Hemanta KV, Sunitha RM. Activity of polyherbal extract against bacteria causing skin infections in diabetic patients. Asian J Pharm Clin Res 2017; 10(5): 147-149.
3. Anderson SE, Meade BJ. Potential health effects associated with dermal exposure to occupational chemicals. Environ Health Insights 2014; 8(1): 51-62.
4. Bader MS. Diabetic foot infection. Am Fam Physician 2008; 78(1): 71-79.
5. Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J of Microbio 2000; 31:247-256.
6. Djeussi ED, Noumedem JAK, Seukep AJ, Fankam G A, Voukeng KI,Tankeo BS, Nkuete HLA and Kuete V. Antibacterial activities of selected edible plants extracts against multidrug-resistant gram-negative bacteria. BMC Compl and Alt Med 2013, 13:164.
7. Mattana CM, Satorres, SE, Escobar F, Sabini C, Sabini L, Fusco M, Alcaraz LE 2012. Antibacterial and cytotoxic activities of Acacia aroma extracts. Emir. J. Food Agric. 24 (4): 308-313.
8. Thamizhvanan K, Kumuda P, Nandakishore R. Antihelmintic and antimicrobial activity of petroleum ether extract of Yucca gloriosa L. whole plant. Int J Preclin Pharmceutical Res 2012; 3(1); 20-22.
9. Sinha J. Medicinal plants of Manipur. Mass and Sinha Manipur Cultural Integration Conference. Imphal 2009.
10. Majumder K, Dutta BK, Roy D. Inventory and status of medicinal trees of Tripura. Ind Med Plant editor, P. C. Trivedi. Avishkar publishers, Distributors. Jaipur 2009; pp 93- 123.
11. Bhardwaj S, Gakhar SK. Ethnomedicinal plants used by the tribals of Mizoram to cure cuts & wounds. Ind J Trad knowledge 2005; 4(1): 75- 80.
12. Bhattacharya D, Samanta S, Mukherjee A, Santra CR, Ghosh AN, Niyogi SK, Karmakar P. Antibacterial Activities of Polyethylene Glycol, Tween 80 and Sodium Dodecyl Sulphate Coated Silver Nanoparticles in Normal and Multi-Drug Resistant Bacteria. J Nanosci and Nanotech 2012; 12: 1–9.
13. Demetrio LVJ, Jeannie IA, Juliana JMP, Esperanza CC. Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria. Asian Pac J Trop Biomed 2015; 5(7): 532-540.
14. Pramanik A, Laha D, Bhattacharya D, Pramanik P, Karmakar, P. A novel study of antibacterial activity of copper iodide nanoparticle mediated by DNA and membrane damage. Colloids and Surfaces B: Biointerfaces 2012; 96: 50– 55.
15. Gupta RN, Viswas K, Pathak M, Parihar SS, Gupta A. Antibacterial activities of ethanol extracts of plants used in folk medicine. Int J Res Ayur Pharm 2010; 1(2); 529-535.
16. Kumar S, Nancy, Singh D, Kumar V. Evaluating the antibacterial activity of plant extracts against bacterial pathogens. J Drug Delivery Therapeutics 2012; 2(4); 182-185.
17. Cabiscol E, Tamarit J, Ros J. Oxidative stress in bacteria and protein damage by reactive oxygen species. Int. Microbiol 2000; 3; 3-8.
18. De Orue Lucana DO, Wedderhoff I, Groves MR. ROS mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron based oxidative stress. J Sig Trans 2012; doi: 10.1155/2012/605905.
Statistics
286 Views | 185 Downloads
How to Cite
1.
Sil S, Saha S, Karmakar P. REACTIVE OXYGEN SPECIES AS POSSIBLE MEDIATOR OF ANTIBACTERIAL ACTIVITY OF PARKIA JAVANICA, AGAINST BACTERIAL SPECIES PREDOMINANTLY FOUND IN CHRONIC WOUND. JDDT [Internet]. 14Jan.2018 [cited 16Jan.2021];8(1):43-7. Available from: http://jddtonline.info/index.php/jddt/article/view/1657