DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ELLAGIC ACID IN ANTIDIABETIC HERBAL FORMULATION

  • Satyendra Shrivastava Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • P K Dubey Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • B Shrivastava School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India
  • Pankaj Sharma School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

Abstract

A simple, sensitive, precise, rapid and reliable HPTLC method for the estimation of ellagic acid in marketed herbal formulation, Glucomap tablet was developed. In this method, precoated Silica Gel F254 Plates were used as stationary phase and Toluene: ethyle acetate: formic acid: methanol (3:3:8:2 v/v) as mobile phase. Developed chromatogram was scanned at 280 nm, the wavelength of maximum absorption for ellagic acid. The aptness of developed HPTLC method for estimation of ellagic acid was established by validating it as per the ICH guidelines. The content of ellagic acid in crude drug Terminalia arjuna and polyherbal formulation was also studied. The developed method has been successfully applied for the determination of ellagic acid in polyherbal formulation.

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Author Biographies

Satyendra Shrivastava, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

P K Dubey, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

B Shrivastava, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

Pankaj Sharma, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

References

1. Mukharjee PK, 3rd ed. Business Horizons Pharmaceutical Publishers, 2002, New Delhi, 183-219.
2. Rose EN, Namdev KP, Samal PK, Standardization strategies for herbal drugs, RJPT, 2008, 1: 310-312.
3. Sethi PD, CBS Publishers and Distributors, 1996, New Delhi, India, 11-17.
4. ICH, ICH-Q2 (R1), International Conference on Harmonization, 2005, Geneva.
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How to Cite
Shrivastava, S., Dubey, P., Shrivastava, B., & Sharma, P. (2017). DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ELLAGIC ACID IN ANTIDIABETIC HERBAL FORMULATION. Journal of Drug Delivery and Therapeutics, 7(7), 180-182. https://doi.org/10.22270/jddt.v7i7.1628