DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ELLAGIC ACID IN ANTIDIABETIC HERBAL FORMULATION

  • Satyendra Shrivastava Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • P K Dubey Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • B Shrivastava School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India
  • Pankaj Sharma School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India
DOI https://doi.org/10.22270/jddt.v7i7.1628

Abstract

A simple, sensitive, precise, rapid and reliable HPTLC method for the estimation of ellagic acid in marketed herbal formulation, Glucomap tablet was developed. In this method, precoated Silica Gel F254 Plates were used as stationary phase and Toluene: ethyle acetate: formic acid: methanol (3:3:8:2 v/v) as mobile phase. Developed chromatogram was scanned at 280 nm, the wavelength of maximum absorption for ellagic acid. The aptness of developed HPTLC method for estimation of ellagic acid was established by validating it as per the ICH guidelines. The content of ellagic acid in crude drug Terminalia arjuna and polyherbal formulation was also studied. The developed method has been successfully applied for the determination of ellagic acid in polyherbal formulation.

Downloads

Download data is not yet available.

Author Biographies

Satyendra Shrivastava, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

P K Dubey, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

B Shrivastava, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

Pankaj Sharma, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India

References

1. Mukharjee PK, 3rd ed. Business Horizons Pharmaceutical Publishers, 2002, New Delhi, 183-219.
2. Rose EN, Namdev KP, Samal PK, Standardization strategies for herbal drugs, RJPT, 2008, 1: 310-312.
3. Sethi PD, CBS Publishers and Distributors, 1996, New Delhi, India, 11-17.
4. ICH, ICH-Q2 (R1), International Conference on Harmonization, 2005, Geneva.
Crossmark
Statistics
257 Views | 273 Downloads
How to Cite
1.
Shrivastava S, Dubey P, Shrivastava B, Sharma P. DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ELLAGIC ACID IN ANTIDIABETIC HERBAL FORMULATION. JDDT [Internet]. 22Dec.2017 [cited 16May2024];7(7):180-2. Available from: https://jddtonline.info/index.php/jddt/article/view/1628
Issue
Vol 7 No 7 (2017): VOLUME 7, ISSUE 7, 2017 (Special Issue: IPSAPHARM)
Section
Research

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).