DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF OLANZAPINE IN MARKETED FORMULATION

  • Nidhi Jain Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111
  • Ankur Joshi Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111
  • Narendra Vyas Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111
  • Sapna Malviya Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111
  • Anil Kharia Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Abstract

A simple, accurate, precise and rapid stability indicating RP-HPLC method was developed and validated for the analysis of Olanzapine in marketed formulation. Analysis was performed on a C-18 (250mm x 4.60mm, 5 µm) column as stationary phase and using mobile phase which was Potassium di-hydrogen phosphate Buffer (pH 6): Acetonitrile (60:40) (v/v) at a flow rate of 1ml/min with UV detection at 258 nm at constant room temperature. The injection volume was 20 µl and the chromatographic runtime of 5 min was used. Proposed method was found to be linear in the range of 5-25 μg/ml with the correlation coefficient 0.998. The validation and the reliability of proposed method were assessed by recovery study. The recovery of added standards (80%, 100% 120%) was ranging from 99.58% - 100.50%. The robustness of developed method was checked by changing temperature, flow rate and mobile phase ratio.

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Author Biographies

Nidhi Jain, Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Ankur Joshi, Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Narendra Vyas, Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Sapna Malviya, Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Anil Kharia, Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

Modern institute of Pharmaceutical Sciences, Indore (M.P.), India, 453111

References

1. Zhang M, Jia J, Chuan LU, Development and Validation of a liquid chromatography-isotope dilution tandem mass spectrometry for determination of olanzapine in human plasma and its application to bioavailability study, Acta Pharm Sin, 2010, 45, 6, 767-771.
2. Mahmoud AT, Nagiba YH, Nariman AE, Mohamed A, Simultaneous determination of Olanzapine and Fluoxetine hydrochloride in capsules by spectrophotometry, TLC-spectrodensitometry and HPLC, JAR, 2013, 4, 173–180.
3. Raja A, Venkateshwar R J, Development and Validation Of new RP-UPLC method for the Quantitative Determination of Olanzapine in Tablet Dosage form , Asian J Pharm Clin Res, 2013, 6, 3, 178- 181.
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How to Cite
Jain, N., Joshi, A., Vyas, N., Malviya, S., & Kharia, A. (2017). DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF OLANZAPINE IN MARKETED FORMULATION. Journal of Drug Delivery and Therapeutics, 7(7), 121-122. https://doi.org/10.22270/jddt.v7i7.1607