SPECTROPHOTOMETRIC AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHODS FOR SIMULTANEOUS DETERMINATION OF ATORVASTATIN AND PIOGLITAZONE IN COMBINED TABLET DOSAGE FORM

  • Tahir Nizami Smriti College of Pharmaceutical Education, Indore, India
  • Birendra Shrivastava School of Pharmaceutical Sciences, Jaipur National University, Jagadapura, Jaipur, Rajasthan, India
  • Pankaj Sharma School of Pharmaceutical Sciences, Jaipur National University, Jagadapura, Jaipur, Rajasthan, India
  • G N Darwhekar Acropolis Institute of Pharmaceutical Education and Research, Indore (Madhya Pradesh), India
  • Praveen Sharma Acropolis Institute of Pharmaceutical Education and Research, Indore (Madhya Pradesh), India

Abstract

Simple, accurate, precise, and sensitive ultraviolet spectrophotometric and reversed-phase high-performance liquid chromatographic (RP-HPLC) methods for simultaneous estimation of Atorvastatin (ATOR) and Pioglitazone (PIO) in combined tablet dosage form have been developed and validated. The spectroscopic methods employs formation and solving of simultaneous equation at 247 nm and 267 nm as 2 wavelengths for estimation of ATOR and PIO respectively (method 1) While method 2 involves formation of Q- absorbance equation at 233 nm (isoabsorptive point) and 267 nm (λ max of Pioglitazone) with methanol as solvent. Beer’s law is obeyed in the concentration range of 5.0–50.0 mcg/mL for ATOR and PIO, respectively. The RP-HPLC method uses HPLC system with a Phenomenex Luna C18 (5 mm x 25cm x 4.6mm i.d) using Methanol, acetonitrile and potassium dihydrogen phosphate buffer, pH 2.5 adjusted with orthophosphoric acid (60:20:20 v/v) at a flow rate of 1.0 mL/min at ambient temperature as the mobile phase. The detection was carried out using an ultraviolet detector set at 233 nm. For the HPLC method, Beer’s law is obeyed in the concentration range of 5.0-50.0 µg/ml for ATOR and PIO, respectively. LOD values for ATOR and PIO were found to be 57.12µg/ml and 12.01 µg /ml respectively. All the methods have been successfully applied for the analysis of the drugs in a pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.1-4

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Author Biographies

Tahir Nizami, Smriti College of Pharmaceutical Education, Indore, India
Smriti College of Pharmaceutical Education, Indore, India
Birendra Shrivastava, School of Pharmaceutical Sciences, Jaipur National University, Jagadapura, Jaipur, Rajasthan, India
School of Pharmaceutical Sciences, Jaipur National University, Jagadapura, Jaipur, Rajasthan, India
Pankaj Sharma, School of Pharmaceutical Sciences, Jaipur National University, Jagadapura, Jaipur, Rajasthan, India
School of Pharmaceutical Sciences, Jaipur National University, Jagadapura, Jaipur, Rajasthan, India
G N Darwhekar, Acropolis Institute of Pharmaceutical Education and Research, Indore (Madhya Pradesh), India
Acropolis Institute of Pharmaceutical Education and Research, Indore (Madhya Pradesh), India
Praveen Sharma, Acropolis Institute of Pharmaceutical Education and Research, Indore (Madhya Pradesh), India
Acropolis Institute of Pharmaceutical Education and Research, Indore (Madhya Pradesh), India

References

1. Munson JW, Pharmaceutical Analysis- Modern Methods, 2011, 3, 16-17.
2. Sharma BK, Instrumental methods of chemical analysis, 24th Edition, Goel Publishing house Meerut, 2005, 311, 266-291.
3. Snyder LR, Kirkland JJ, Glajch JL, Practical HPLC method development, 2nd Edition, Wiley Interscience Publication, 1997, 966-986.
4. Indian Pharmacopoeia, 1996, 1, 347-348.
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How to Cite
Nizami, T., Shrivastava, B., Sharma, P., Darwhekar, G., & Sharma, P. (2017). SPECTROPHOTOMETRIC AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHODS FOR SIMULTANEOUS DETERMINATION OF ATORVASTATIN AND PIOGLITAZONE IN COMBINED TABLET DOSAGE FORM. Journal of Drug Delivery and Therapeutics, 7(7), 116-117. https://doi.org/10.22270/jddt.v7i7.1605