A NOVEL CLASS OF PHOTOTRIGGERABLE LIPOSOMES CONTAINING PACLITAXEL FOR THE TREATMENT OF SKIN CANCER
Success of nanocarriers-mediated drug delivery solely depends on delivery of therapeutics to a specified target. Secondly therapeutically active amount of drug should be released within defined space and time (triggered release). Recently, we formulated a novel class of photo-triggerable liposomes prepared from soy lecithin (SPC), cholesterol (CHOL) and photosensitive agent ketoprofen that can efficiently released entrapped paclitaxel upon UV light treatment. To explore these formulations for in vivo applications, we have examined the effect of released anticancer drugs on cellular toxicity. Liposomes were loaded with paclitaxel and biophysical properties (including liposome size and stability) and paclitaxel encapsulation efficiency of the liposomes were determined. Subsequently, the effect of UV light treatment on paclitaxel release, and cellular toxicity by released paclitaxel were examined. Since liposomes using the 5:1 molar ratio of SPC and CHOL, showed highest encapsulation of paclitaxel, these formulations were investigated further. UV light treatment of co-cultures containing paclitaxel loaded liposomes and cells (SK-MEL-2) resulted in improved cell killing as compared to untreated samples. These phototriggerable liposomes described here may provide a platform for future drug delivery applications.Â
2. Bisby RH, Mead C, Morgan CG, Active uptake of drugs into photosensitive liposomes and rapid release on UV photolysis, FEBS Lett, 1999, 463, 165-168.
3. Kono K, Yoshino K, Takagishi T, Antiinflammatory effect of retrovirally transfected interleukin-10 on monosodium urate monohydrate crystal induced acute inflammation in murine air pouches, J Control Release, 2002, 80, 321-332.
4. Gerasimov OV, Boomer JA, Qualls MM, Synthesis and characterization of oligomaltose grafted lipids with application to liposomes, Adv Drug Deliv Rev, 1999, 38, 317-338.
5. Gerasimov OV, Schwan A, Thompson DH, Acid catalysedplasminylcholine hydrolysis and its effect on bilayer permeability: A quantitative study, Biochim Biophys Acta, 1997, 1324, 200-214.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).