HPTLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CINNAMALDEHYDE IN POLYHERBAL FORMULATION WITH ANTI-DIABETIC PROPERTY

  • Satyaendra K Shrivastava Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • P K Dubey Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • Pankaj Sharma School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
  • B Shrivastava School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
DOI https://doi.org/10.22270/jddt.v8i1.1553

Abstract

In this research article HPTLC method for the estimation of cinnamaldehyde content in cinnamomum zeylanicum, laboratory batches (MC-I, MC-II, MC-III) and marketed formulation (MCM) was developed. In this method, precoated Silica Gel F254 plates were used as stationary phase and Toluene: ethyl acetate: formic acid (8:1:1v/v) as mobile phase. Developed chromatogram was scanned at 295 nm, the wavelength of maximum absorption for cinnamaldehyde. The aptness of developed HPTLC method for estimation of cinnamaldehyde was established by validating it as per the ICH guidelines. The developed method has been successfully used for the estimation of cinnamaldehyde in polyherbal anti-diabetic formulation.

Keywords: Polyherbal formulation, Cinnamaldehyde, Cinnamomum zeylanicum, HPTLC.

Downloads

Download data is not yet available.

Author Biographies

Satyaendra K Shrivastava, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
P K Dubey, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Pankaj Sharma, School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
B Shrivastava, School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)

References

1. Govil GN, Singh VK: Recent Progress in Medicinal Plants: Standardization of herbal/Ayurvedic formulations, Studium Press LLC Publisher, 2009; 24:46.
2. Rose EN, Nmedo KP and Samal PK. J. Pharm. Tech., 2008; 1:310-312.
3. Organisation Mondiale De La Sante.: Quality control methods for medicinal plant materials, 559, (1) Original English, World Health Organisation, 1992:159.
4. Sethi PD. HPTLC: High Performance Thin Layer Chromatography, CBS Publishers and Distributors, New Delhi, India, 1996; 11-17.
5. ICH. (2005). Validation of analytical procedures: text and methodology. ICH-Q2 (R1), International Conference on Harmonization: Geneva.
Statistics
477 Views | 340 Downloads
How to Cite
1.
Shrivastava S, Dubey P, Sharma P, Shrivastava B. HPTLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CINNAMALDEHYDE IN POLYHERBAL FORMULATION WITH ANTI-DIABETIC PROPERTY. JDDT [Internet]. 14Jan.2018 [cited 21Jan.2022];8(1):70-3. Available from: http://jddtonline.info/index.php/jddt/article/view/1553
Issue
Vol 8 No 1 (2018): VOLUME 8, ISSUE 1, Jan-Feb 2018
Section
Research

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).