HPTLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CINNAMALDEHYDE IN POLYHERBAL FORMULATION WITH ANTI-DIABETIC PROPERTY

  • Satyaendra K Shrivastava Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • P K Dubey Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
  • Pankaj Sharma School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
  • B Shrivastava School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)

Abstract

In this research article HPTLC method for the estimation of cinnamaldehyde content in cinnamomum zeylanicum, laboratory batches (MC-I, MC-II, MC-III) and marketed formulation (MCM) was developed. In this method, precoated Silica Gel F254 plates were used as stationary phase and Toluene: ethyl acetate: formic acid (8:1:1v/v) as mobile phase. Developed chromatogram was scanned at 295 nm, the wavelength of maximum absorption for cinnamaldehyde. The aptness of developed HPTLC method for estimation of cinnamaldehyde was established by validating it as per the ICH guidelines. The developed method has been successfully used for the estimation of cinnamaldehyde in polyherbal anti-diabetic formulation.

Keywords: Polyherbal formulation, Cinnamaldehyde, Cinnamomum zeylanicum, HPTLC.

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Author Biographies

Satyaendra K Shrivastava, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India
P K Dubey, Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Swami Vivekanand College of Pharmacy, Khandwa Road, Indore (M.P.), India

Pankaj Sharma, School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
B Shrivastava, School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)
School of Pharmaceutical Sciences, Jaipur National University, Jaipur (Rajasthan)

References

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1.
Shrivastava S, Dubey P, Sharma P, Shrivastava B. HPTLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CINNAMALDEHYDE IN POLYHERBAL FORMULATION WITH ANTI-DIABETIC PROPERTY. JDDT [Internet]. 14Jan.2018 [cited 17Oct.2021];8(1):70-3. Available from: http://jddtonline.info/index.php/jddt/article/view/1553