SUPERFICIAL ANTICANDIDOSIC ACTIVITY OF LEAVES OF COMBRETUM RACEMOSUM P. BEAUV. (COMBRETACEAE) EXTRACTS

  • Kamou Richard KAMOU 1University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast 2University Felix Houphouët-Boigny of Cocody, UFR of Pharmaceutical and Biological Sciences, Laboratory of Bacteriology-Virology, 22 BP 582 Abidjan 22, Ivory Coast
  • Yessé Zinzendorf NANGA University Felix Houphouët-Boigny of Cocody, UFR of Pharmaceutical and Biological Sciences, Laboratory of Bacteriology-Virology, 22 BP 582 Abidjan 22, Ivory Coast
  • Abou OUATTARA University Jean Lorougnon Guede of Daloa, UFR Agroforestry, Department of Biochemistry and microbiology, BP 150 Daloa, Ivory Coast
  • Sansan Philippe KAMBOU University Felix Houphouët-Boigny of Cocody, UFR of Médical Sciences, Laboratory of Médical Biochemistry, 01 BP 240 Abidjan 01, Ivory Coast
  • Gouéh GNAHOUE Teaching Training School (ENS) of Cocody, Laboratory of Biochemistry-Microbiology (SVT Section), 08 BP 10 Abidjan 08, Ivory Coast
  • Calixte BAHI University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast
  • Adama COULIBALY University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast

Abstract

This preliminary work consists in evaluating the superficial anticandidic activity of the leaves of Combretum racemosum P. Beauv in humans. To this end, we first demonstrated bioactive compounds of five (5) crude extracts (aqueous and hydro-organic 70%) from C. racemosum. Then, and finally, we have separately evaluated the superficial anticandidic activity of these extracts on Candida albicans, a species of genus Candida, mostly encountered in superficial human candidiasis. C. albicans was found to be sensitive to the five extracts tested. This partly justifies the use of C. racemosum in traditional medical practice against superficial microbial diseases. For these extracts, the Inhibitory Minimum Concentrations (MIC) recorded ranged from 7.8125 mg/mL to 62.550 mg/mL; While Fungicide Minimum Concentrations (CMF) range from 31.25 ± 0.00 mg/mL to 62.550 mg/mL. Concentrations for Fifty Percent Inhibition (IC50) range from 0.930 mg/mL to 3.861 mg/mL. The hydroethanolic macerated extract 70% (Eeth 70%) is the most active fraction on C. albicans (CMF = 7.8125 ± 0.00 mg/mL, IC50 = 0.941 ± 0.009 mg/mL) compared to the total of the five crude extracts tested. The preliminary phytochemical screening of the five crude extracts revealed the presence of alkaloids, saponins, terpenoids steroids and tannins (catechics and gallics) at various levels of concentration in our study. Eeth 70%, the most active extract in our work, contains in medium concentrations the same active ingredients cited above.  Finally, we note that the hydroethanolic macerated extract 70% is the most active fractions in this work. It may, however, subject to toxicological studies, be used in the treatment of theskin, the cutaneous appendages and the mucous membranes in humans.

Keywords: Combretum racemosum, phytochemical screening, superficial anticandidic

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Author Biographies

Kamou Richard KAMOU, 1University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast 2University Felix Houphouët-Boigny of Cocody, UFR of Pharmaceutical and Biological Sciences, Laboratory of Bacteriology-Virology, 22 BP 582 Abidjan 22, Ivory Coast

1University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast

2University Felix Houphouët-Boigny of Cocody, UFR of Pharmaceutical and Biological Sciences, Laboratory of Bacteriology-Virology, 22 BP 582 Abidjan 22, Ivory Coast

Yessé Zinzendorf NANGA, University Felix Houphouët-Boigny of Cocody, UFR of Pharmaceutical and Biological Sciences, Laboratory of Bacteriology-Virology, 22 BP 582 Abidjan 22, Ivory Coast

University Felix Houphouët-Boigny of Cocody, UFR of Pharmaceutical and Biological Sciences, Laboratory of Bacteriology-Virology, 22 BP 582 Abidjan 22, Ivory Coast

Abou OUATTARA, University Jean Lorougnon Guede of Daloa, UFR Agroforestry, Department of Biochemistry and microbiology, BP 150 Daloa, Ivory Coast

University Jean Lorougnon Guede of Daloa, UFR Agroforestry, Department of Biochemistry and microbiology, BP 150 Daloa, Ivory Coast

Sansan Philippe KAMBOU, University Felix Houphouët-Boigny of Cocody, UFR of Médical Sciences, Laboratory of Médical Biochemistry, 01 BP 240 Abidjan 01, Ivory Coast

University Felix Houphouët-Boigny of Cocody, UFR of Médical Sciences, Laboratory of Médical Biochemistry, 01 BP 240 Abidjan 01, Ivory Coast

Gouéh GNAHOUE, Teaching Training School (ENS) of Cocody, Laboratory of Biochemistry-Microbiology (SVT Section), 08 BP 10 Abidjan 08, Ivory Coast

Teaching Training School (ENS) of Cocody, Laboratory of Biochemistry-Microbiology (SVT Section), 08 BP 10 Abidjan 08, Ivory Coast

Calixte BAHI, University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast

University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast

Adama COULIBALY, University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast

University Felix Houphouët-Boigny of Cocody, UFR Biosciences, Laboratory of Pharmacodynamic-Biochemistry, 22 BP 582 Abidjan 22, Ivory Coast

References

1. Scott DW, Miller WH and Griffin CE: Muller & Kirk's Small Animal Dermatology. 6th Edition Saunders 2001, 1552p.
2. Coulaud JP: Infections parasitaires et fongiques au cours du SIDA. Abstract, Euromedecine Montpellier 1987, 535-536.
3. Vandeputte P: Mécanismes moléculaires de la résistance aux antifongiques de Candida glabrata. Université d’angers.Thèse de doctorat, 2008, n°930.
4. Sellami H et ayadi H: Etude de la sensibilité des différentes espèces de Candida. Rev. Tun Infectiol 2008; 2 (2):1-79.
5. Piens MA, Monbrison de F et Picot S: Etude de la sensibilité des levures aux antifongiques en pratique médicale, let Infection 2003, XVIII n°6: 222-226.
6. Djohan VKE, Angora AH et Vanga-Bosson et coll: Sensibilité in vitro des souches de Candida albicans d’origine vaginale aux antifongiques à Abidjan (Côte d’Ivoire). J. mycol. Méd. 2011; 22(2):129-133
7. Tra Bi FH, Kouamé NG, Favel A et Fallague K : Activité antifongique de quelques plantes de la flore ivoirienne. Sciences et Nature 2007; 4(2):117-122.
8. Zhang W and Björnl: The effect of ultraviolet radiation on the accumulation of medicinal compounds in plants Fitoterapia 2009; 80(4):207-218.
9. Borgers M, Degreef H and Cauwenbergh G: Fungal infections of the skin: infection process and antimycotic therapy Curr Drug Targets 2005; 6:849–862.
10. Eholie SP: Etude des infections opportunistes au cours du SIDA, à propos de 262 cas diagnostiqués, Thèse. Dipl. d’Etat Médecine, Abidjan 1993, n°1517: 81p.
11. De Souza C, Koumaglo K et Gbeassor M : Evaluation des propriétés antimicrobiennes des extraits totaux de quelques plantes médicinales. Rev. Méd. et pharm. Afr. 1995, Numéro spécial, 103-112.
12. Dosso M, Aissi H, Faye K, Saracino J et Kadio A: Evaluation de la sensibilité des bactéries hospitalières en zone tropicale à propos de 2543 souches de bacilles gram négatif isolées au CHU de Cocody, Méd. Mal. Infec. 1986,; 6(4):241-244.
13. Matsuda Y, Sugiura K, Hashimoto T, Ueda A, Konno Y and Tatsumi Y: Efficacy coefficients determined using nail permeability and antifungal activity in keratin- containing media are useful for predicting clinical efficacies of topical drugs for onychomycosis, PLoS ONE, 2016, 11p.
14. Bennet MD , Bhandol P and Leitch IJ: Nuclear DNA amounts in angiosperms and their modern Uses 807 new estimates. Annals of Botany 2000; 86:859-909.
15. Wingard JR. and Leather H: A new era of antifungal therapy. Biology of Blood and Marrow Transplantation 2004; 10:73-90.
16. Odds FC, Brown AJP, Gow NAR: Antifungal agents: Mechanisms of action. Trends in Microbiology 2003; 11:272-279.
17. Li L, Naseem S, Sharma S and Konopka JB: Flavodoxin-Like Proteins Protect Candida albicans from Oxidative Stress and Promote Virulence. PLoS Pathog. 2015; 11(9).
18. Dupont B, Dromer F and Improvisi L: The problem of azole resistance in Candida. J. Mycol. Med. 1996, 6p.
19. Ferrari J: Contribution à la connaissance du métabolisme secondaire des Thymelaeaceae et investigation phytochimique de l’une d’elles: Gnidia involucrate Steud, ex A. Rich., Thèse de doctorat, Lausanne 2002.
20. Bahorun T: Substances naturelles actives: la flore mauricienne, une source d’approvisionnement potentielle. Food and agricultural resarch council 1997, 83-94
21. Marfak A: Radiolyse gamma des flavonoïdes, étude de leur réactivité avec les radicaux issus des alcools: formation de depsides, Thèse de doctorat, Limoges 2003, 181p.
22. World Organization of Health: A report of the consultation meeting on traditional and modern medicine: Harmonizing two approaches, Beijing, China 2000; 22-26.
23. De Morais Lima GR., De Sales IR, Caldas Filho MR, De Jesus NZ, De Sousa FH, Barbosa-Filho JM, Guedes Silveira Cabral A, Souto Lope A, Fechine Tavares J et Batista LM : Bioactivités du genre Combretum (Combretaceae): Un examen, en molécules 2012; 17:9142-9206.
24. Stace C A: Les familles et Généralités des plantes vasculaires. Springer, Berlin Heidelbergerberg 2007; 9:67-82.
25. Burkill HM: The useful plants of West Tropical Africa. Royal Botanical Gardens, Kew 2000; 5:378p.
26. Oghenejobo M, Oghenejobo BUS, Uvieghara KE and Omughele E: Phytochemical screening and antimicrobial activities of the fractionated leaf extract of Combretum racemosum, Scholars. Academic Journal of Pharmacy 2014; 3(6):455-462.
27. Burkill HM: The useful plants of West Tropical Africa. Royal Botanical garden 1985; 5:378p.
28. Mpona M: Cours de Mycologie, 3 ème graduat ISTM/KIN/Labo Inétit 2008.
29. Fidel PL and Sobel JD: Immunopathogenesis of recurrent vulvovaginal candidiasis. Clin. Microbiol. Rev. 1996; 9:335-348.
30. Béné K, Camara D, Fofié N`GBY et Zirihi GN: Etude ethnobothanique, activité antifongique in-vitro sur Candida albicans et toxicité sur les cellules HFF de HIrrizoniaabissinica Oliv. (Simaroubaceae), une plante de la pharmacopée ivoirienne. Journal of Applied Biosciences 2015; 94:8815-8824.
31. Zirihi GN, Kra AKM et Guédé-Guina F : Evaluation de l’activité antifongique de Microglossa pyrifolia (Larmarck) O kuntze (Asteraceae) “pymi” sur la croissance in vitro de Candida albicans. Revue Méd. Pharm. Afr. 2003; 17:11-189.
32. Ackah AJ, Kra, AM, Zirihi GN et Guédé-Guina F: Evaluation of the antifungal activity of TEKAM, évaluation de l'activité antifongique de tekam, un extrait de plante, sur la croissance in vitro de candida albicans. Rev. Ivoir. Sci. Technol. 2008; 11:119-129.
33. A. GUEDE: Etude des effets physiologiques d’un extrait aqueux de Landolphia hirsuta (Apocynacée), traditionnellement utilisé comme antihémorroïdaire et essai de formulation de suppositoires à effet antihémorroïdaire, Thèse de doctorat en pharmacie – UFR de Pharmacie d’Abidjan, N° 815/03, 2003, 200p.
34. Mangambu MJ, De D, Mushagalusa KF et Kadima NJ: Contribution à l’étude phytochimique de quelques médicinales antidiabétiques de la ville de Bukavu et ses environs (Sud-Kivu, R. D. Congo). Journal of Applied Biosciences 2014; 75: 6211-6220.
35. Yayé YG, Ackah JAAB, Kra AKM and Djaman JA: Anti-Fungal Activity of Different Extracts of Terminalia Mantaly H. Perrier on the in vitro Growth of Aspergillus fumigatus. Eur. J. Sc. Res. 2012, 82: 132-138.
36. Ackah JAAB, Yayé YG, Yapi HF, Kra AKM and Djaman AJ: Antifungal activity of Terminalia mantaly on the in vitro Growth of Cryptococcus neoformans. International Journal of Biochemistry Research & Review 2013; 3(1):63-73.
37. Ackah AJ, Kra AM, Zirihi GN et Guédé-Guina F: Evaluation de l'activité antifongique de tekam, un extrait de plante, sur la croissance in vitro de candida albicans. Rev. Ivoir. Sci. Technol, 2008c; 11:119-129.
38. Guédé-Guina F, Wangah-Manda M, Bonga GM et De Sousa C: Activité antimicrobienne d’un extrait végétal contre les germes opportunistes au cours du SIDA. Rev. Méd. Pharm. Afr. 1995; 9(l):l3-19.
39. Kra AKM: Evaluation et amélioration par séquençage chromatographique d’une action antifongique de MISCA contre Aspergillus fumigatus. Thèse de doctorat de 3 ème cycle; Option: Pharmacologie-Microbiologie. Université de Cocody, U.F.R. Biosciences. Abidjan, Côte d`Ivoire, 2001, 116p.
40. Harbone JP: Méthodes phytochimiques, 2nd Ed., Dakota du Nord Oxford Université Presse, Londres 1991.
41. Onocha PA, Audu EO, Ekundayo O and Dosumu OO: Phytochemical and antimicrobial properties of extracts of Combretum racemosum. Acta Horticulturae 2005a; 675:2-97.
42. Okwuosa CN, Achukwu PUO, Azubike NC et Abah AIE: Effet de protection des extraits de feuilles de Combretum racemosum P. Beauv (Combretaceae) sur la Pancytopaénie et la lésion hépatique induite par Cyclophospahamide chez les rats mâles. Research Journal of Pharmacology 2012; 6: 30-34.
43. Kamou KR, Ouattara K, Bagré I, Gnahoué G, Ouattara A et Coulibaly A : Screening phytochimique et activités antifongiques d´extraits de Combretum racemosum (P. Beauv.) sur cinq souches fongiques cliniques. Thèse de doctorat en Biotechnologies, UFR des Biosciences d´Abidjan. Journal Mondial de la Recherche Pharmaceutique, 2016, 5(10): 119-126.
44. Cowan MM: Plant products as antimicrobial agents. Clin. Microbiol. Rev. 1999; 12(4):564-582.
45. Chodera A, Dabrowska A, Sloderbach A, Skrzypczak L, Budzianowski J: Effect of flavonoid fractions of Solidago virgaureaL on dieresis and levels of electrolytes. Acta Pol Pharm. 1991: 48: 35-37.
46. Rizvi SH, Shoeb A, Kapil RS, Popli PS: Two diuretic triterpenoids from Antidermamenasu. Phytochemistry 1980, 19(11): 2409-2410.
47. Mcgaw LJ, Rabe T, Sparg SG, Jager AK, Eloff JN and Van Stadan J: An investigation on the biological activity of Combretum species. Journal of Ethnopharmacology 2001; 75:45-50.
48. Onocha PA, Audu EO, Ekundayo O and Dosumu OO: Phytochemical and antimicrobial properties of extracts of Combretum racemosum. Acta Horticulturae 2005b, 675: 2-97.
49. Okwuosa OMTB, Chukwura EL, Chukwuma GO, Enweani IB, Agbakoba NR, Chukwuma CM, Manafa PO et Umedum CU: Activités phytochimiques et antifongiques des feuilles et raciness d’Uvaria chamae, des feuilles et écorces de Spondias mombi et des feuilles de Combretum racemosum, suppl. Afr. J. Med. Sci. 2012; 41: 99-103.
50. Faizi S, Rashid M, Alik RA, Ahmed S, Khan SA, Aqeel AA, Bibi N et Ahmed SA: Évaluation de propriété antimicrobienne de Polyalthia longifolia var. Pendula: isolement d'une lactone comme l'agent antibactérien actif de l'extrait d'éthanol de la tige. Phytother, Res. 2003; 17(10):1177-1181.
51. Scalbert: Propriétés antimicrobiennes des tannins, Phytochimie 1991, 30: 3875-3883.
52. Cimanga K, Kambu K, Tona L and Hermans N et al.: Cytotoxicity and in vitro susceptibility of Entamoeba histolytica to Morinda morindoides leaf extracts and its isolated constituents. Journal of Ethnopharmacology 2006; 107: 83-90.
53. Mankele R, Ouamba JM, Abena AA et Yalaf A: Étude des effets de Morinda morindoïdes (Back) sur le système immunitaire de l’homme. Phytothérapie 2006; 4(2): 68-73.
54. Koechlin-Ramonatxo C: Oxygen, oxidative stress and antioxidant supplementation, or another way of nutrition in respiratory diseases. Nutr. Clin. et Métab. 2006; 20:165-177.
55. Suhaj M: Spice antioxidants isolation and their antiradical activity: a review. J. Food Compos. and Analys. 2006, 19: 531–537.
56. Scazzocchio F, Comata MF, Tomassini L and Palmery M, Antibacterial activity of Hydrastis canadensis extract and its major isolated alkaloids. Planta medica 2001; 67(6):561-564.
57. Muster D, Lotfi BS: Thérapeutique médicale buccodentaire: moyens et methods Publié par Elsevier Masson, ISBN 2842995651, 9782842995652, 2004, 290p.
58. Wagner H: Pharmazeutische Biologie. Drogen und irhe inhaltsstoffe, Gustav Fisher Verfag, Sturtgart-New-York 1993, 522p
59. Thangara JHS, Adjei O, Allen BW and Portaels F et al.: In vitro activity of ciprofloxacin, Sparfloxacin, Ofloxacin, Amikacin and Rifampicin against Ghanian isolates of Mycobacterium ulcerans, agents Chemother. J. Antimicrob. 2000; 45 (2):231-233.
60. Marmonier AA: Introduction aux techniques d’étude des antibiotiques, Bactériologie Médicale, technique usuelles 1990; 227-236.
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KAMOU K, NANGA Y, OUATTARA A, KAMBOU S, GNAHOUE G, BAHI C, COULIBALY A. SUPERFICIAL ANTICANDIDOSIC ACTIVITY OF LEAVES OF COMBRETUM RACEMOSUM P. BEAUV. (COMBRETACEAE) EXTRACTS. JDDT [Internet]. 13Sep.2017 [cited 27Nov.2021];7(5):53-0. Available from: http://jddtonline.info/index.php/jddt/article/view/1484