HYPOGLYCAEMIC ACTIVITY OF CENTRATHERUM ANTHELMINTICU IN EXPERIMENTAL ANIMALS

  • Ramji Swarnkar Institute of Pharmacy, Shri Venkateshwara University, Gajroula, Uttar Pradesh,
  • Chandrul Kaushal Institute of Pharmacy, Shri Venkateshwara University, Gajroula, Uttar Pradesh,
  • S K Jain Institute of Pharmacy, Bundelkhand University, Jhansi,

Abstract

The Effect of Ethanolic extracts of Centratherum anthelminticum on Diabetic rats Assessment of the antihyperglycemic effect of ethanolic extract of Centratherum anthelminticum seeds in normal and alloxan induced diabetic rats: The animals will be divided into nine groups and each group consisted of six rats. Group 1 as normal control. Group 2 as the diabetic control. Groups 3, 4 and 5 are normally treated with 0.25 g, 0.50 g and 0.75 g/kg BW of CAEt, respectively. Groups 6, 7 and 8 are diabetic rats treated with 0.25 g, 0.50 g, and 0.75 g/kg BW of CAEt, respectively. Group 9 are diabetic rats which received glibenclamide (0.02 g/kg BW) an oral hypoglycemic agent dissolved in distilled water. Normal control and the diabetic control rats are fed distilled water alone. After an overnight fast, the plant extract suspended in distilled water will feed to the rats by gastric intubation using a force feeding needle. Blood samples are collected for the measurement of blood glucose from the tail vein at 0, 1, 2, 3, 4, 5 and 6 h after the administration of plant extract and blood glucose levels are determined by Glucose oxidase – peroxidase method. The phytochemical analysis will be carried out in the ethanolic extract by different methods of phytochemical analysis.

Keywords: Hypoglycaemia, Diabetes mellitus, Centratherum anthelminticum

The effect of Ethanolic extracts of Centratherum anthelminticum on Diabetic rats Assessment of antihyperglycemic effect of ethanolic extract of Centratherum anthelminticum seeds in normal and alloxan induced diabetic rats: The animals will be divided in to nine groups and each group consisted of six rats. Group 1 as normal control. Group 2 as the diabetic control. Groups 3, 4 and 5 are normal treated with 0.25 g, 0.50 g and 0.75 g/kg bw of CAEt, respectively. Groups 6, 7 and 8 are diabetic rats treated with 0.25 g, 0.50 g and 0.75 g/kg bw of CAEt, respectively. Group 9 are diabetic rats which received glibenclamide (0.02 g/kg bw) an oral hypoglycemic agent dissolved in distilled water. Normal control and the diabetic control rats are fed distilled water alone. After an overnight fast, the plant extract suspended in distilled water will fed to the rats by gastric intubation using a force feeding needle. Blood samples are collected for the measurement of blood glucose from the tail vein at 0, 1, 2, 3, 4, 5 and 6 h after the administration of plant extract and blood glucose levels are determined by Glucose oxidase – peroxidase method.Phytochemical analysis will carried out in the ethanolic extract by different methods of phytochemical analysis.

Keywords: Hypoglycaemia, Diabetes mellitus, Centratherum anthelminticum

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Author Biographies

Ramji Swarnkar, Institute of Pharmacy, Shri Venkateshwara University, Gajroula, Uttar Pradesh,

Institute of Pharmacy, Shri Venkateshwara University, Gajroula, Uttar Pradesh, India

Chandrul Kaushal, Institute of Pharmacy, Shri Venkateshwara University, Gajroula, Uttar Pradesh,
Institute of Pharmacy, Shri Venkateshwara University, Gajroula, Uttar Pradesh, India
S K Jain, Institute of Pharmacy, Bundelkhand University, Jhansi,
Institute of Pharmacy, Bundelkhand University, Jhansi, India

References

1. Carvalho E. N, Carvalho N. A. S, Ferreira L. M, “Experimental model of induction of diabetes mellitus in rats”, Acta Cir Bras, 2003; (18):268-72
2. Handa S.S, Chawla A.C, Maninder A. “Hypoglycaemic Plants review”. Fitoterapia 1989; (60):195-222.
3. Holman R.R, Turner R.C, “oral agents and insulin in the treatment of NIDDM”, Textbook of diabetes Oxford Black, 1991; 469-470.
4. Ohkubo Y. Kishikawa, H. Araki, E. “intensive insulin therapy prevented the progression of diabetic microvascular complications in Japanese patients with non insulin dependent diabetes mellitus: a randomized prospective six year study”, Diabetes res clin Prac. Pub Med 1995; (28):103.117.
5. Qureshi S.A, Nawaz, A. Udani S.K, Azmi, M.B, “Hypoglycaemic and hypolipidemic activities of Rauwolfia serpentina in alloxan-induced diabetic rats”, Int J Pharmacol, 2009; (5):323-326.
6. World Health Organization, “second report of the WHO Expert committee on diabetes mellitus”, Geneva, Technical Report Series; 1980; 646-666.
7. Zhang, X.F, Tan, B.K.H, “Antihyperglycemic and antioxidant properties of Andrographis paniculata in normal and diabetic rats, clin Exp Pharmacol Physiol; (2002); 27(29):358-363.
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Swarnkar R, Kaushal C, Jain S. HYPOGLYCAEMIC ACTIVITY OF CENTRATHERUM ANTHELMINTICU IN EXPERIMENTAL ANIMALS. JDDT [Internet]. 15Jul.2017 [cited 19May2022];7(4):73-7. Available from: http://jddtonline.info/index.php/jddt/article/view/1472