METHOD DEVELOPMENT AND VALIDATION OF IRBESARTAN CHLORTHALIDONE AND CILNIDIPINE IN THEIR COMBINED TABLET DOSAGE FORM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Rishabh K Dagariya, Rakesh K Jat

Abstract


The present work describes a validated reverse phase high performance liquid chromatographic method for simultaneous estimation of Irbesartan chlorthalidone and cilnidipine in tablet dosage form. The quantification was carried out using C18 column (250 x 4.6mm, 5μm) and mobile phase comprised of Buffer, Acetonitrile and TEA in a proportion of 80:20:0.1 %v/v/v. The flow rate was 1.0 ml/min and the eluent was monitored at 222 nm. The selected chromatographic conditions were found to effectively separate Irbesartan Chlorthalidone and cilnidipine were 3.807 min, 4.667 min, and 6.887 min respectively. Linearity was found to be in the range of 30-90 μg/ml, 1.25-3.75 μg/ml and 1-3 μg/ml for Irbesartan Chlorthalidone and cilnidipine respectively. The percentage recoveries of all the drugs were found to be 99.27-99.81%, 99.57-99.99% and 99.22-99.44% for Irbesartan, chlorthalidone, and cilnidipine. The proposed method was found to be fast, accurate, precise, and reproducible and can be used for simultaneous estimation of these drugs in a tablet.


References


“Drug profile for Chlorthalidone”, Sep 2015, http://www.drugbank.ca/drugs/DB00310

“Drug profile for Chlorthalidone”, Sep 2015,http://en.wikipedia.org/wiki/ Chlorthalidone

“Drug profile for Cilnidipine”, Sep 2015, en.wikipedia.org/wiki/Cilnidipine.

“Drug profile for Cilnidipine”, Sep 2015, www.drugbank.ca/drugs/DB03614

Niraimathi V, Jerad SA, Kumar SI. Uv Spectrophotometric Methods For The Estimation OF Chlorthalidone In Bulk and Oral Dosage Form. Indo American J. Pharm,2013; 3(9):7160-7167.

Rang and Del’s Pharmacology; 6th Edn; Churchil levingstone Publishers (P) Ltd, New York, 2004, pp 277-285.

Dong WM. Modern HPLC for Practicing Scientists; A Wiley- Inter science publication, USA, 2006, pp 1-9.

Kazakevich Y and LoBrutto R. HPLC for pharmaceutical Scientists; A John Wiley and sons, 2007, pp 1-6.

Mhaske RA, Sahsrabhdhe S, mhaske AA, “RP-HPLC Method For Simultataneous Determination of Irbesartan, Losartan, Hydro-Chlorothiazide And Chlorthalidone–Application To Commercially Available Drug Products” Int. j. pharm. Sci. and res., 2014; 1116-1123

Gandla K, Rao JS, Kumar JR, “New Stability Indicating Validated RP-HPLC Method for Simultaneous Estimation of Irbesartan and Atorvastatin in Combined Tablet Dosage Forms” Ame. J. pharm. Tech. res., 2015; 5(4):380-390

Gandla K, Suthakaran R, Kandoji P, “Development and Validation of RP-HPLC Method for Simultaneous Estimation of Irbesartan and Simvastatin in Tablet Dosage Form” Ame. J. pharm. And health res., 2014; 2(10):199-205

Patel S, Patel D” Simultaneous Determination of Metoprolol Succinate And Chlorthalidone By UV Spectrophotometric Method.” Pharmagene. 2013; 1(3):39-43

Naazneen S, Sridevi A,” Stability-Indicating RP-HPLC Method For The Simultaneous Estimation of Azilsartan Medoxomil And Chlorthalidone In Solid Dosage Forms. ” Int. J. of Pharm. and Pharm. Sci. 2014; 6(6):236-243

Kalaiselvi P and Lalitha K G, “Development and Validation of RP-HPLC Method For The Simultaneous Estimation of Chlorthalidone And Irbesarton In Pharmaceutical Dosage Form.”Pharmacophore.2014; 5(2):279-286.

Lee KH, Park HJ, Shin CY, Myung CS “Pharmaceutical composition comprising cilnidipine and telmisartan”, European Patents, PCT/KR2009/002539, 2010


Full Text: PDF

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

footer_848
ISSN: 2250-1177  This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License.