OPTIMIZATION OF RIFAMYCIN B USING NOCARDIA MEDITERRANEA (ATCC 13865) BY FED BATCH MODE IN SHAKE FLASKS
The present study was carried out to evaluate the optimum Production of Rifamycin B by using fed- batch mode in the shake flask at the defenit level of different agitation and aeration rate, amongst different carbohydrate in the form of monoshacride. Amongst the various carbohydrate (Arabinose, Fructose, Galactose, Glucose, Glycerol, Maltose, Mannitol, Sorbitol, and Sucrose) sources maximum, the rifamycin (578 Âµgml-1) with 10% glucose level was found as compared to control which was (138 Â± 2.70Âµgml-1).The environmental factors such as incubation temperature. The optimum temperature for maximum antibiotic production (525Âµgml-1) was found to be 29Â°C, initial pH, aeration and agitation rates were also optimized. The optimum rifamycin yield (950 to 1100Âµgml-1) was obtained in 1.0 L fermenter with working volume of 1.5 L at 28 Â°C with initial pH of 6.2 to 6.5. The agitation and aeration were 300 rpm and 0.5 LL-1 min-1respectively.
Keywords: Nocardia mediterranea ATCC 13865, Agitator, Carbohydrate, Rifamycin B, Fed-batch, Optimization.
2. Bapat PM, Wangikar PP, â€œOptimization of Rifamycin B fermentation in shake flasks via a machine-learning-based approachâ€, Biotechnol Bioeng, 2004; 86:201â€“208.
3. El Enshasy HA, El Baz AF, and Ammar EM. â€œSimultaneous production and decomposition of different Rifamycins during Amycolatopsis mediterranei growth in shake flask and in stirred tank bioreactorâ€, Current Research and Educational Topics and Trends in Applied Microbiology A. MÃ©ndez-Vilas,Formatax (Ed.), 2005; 315-21
4. Heinz G. Floss and TinÂWein Yu, â€œRifamycin Mode of Action, Resistance, and Biosynthesisâ€, Chemical Reviews, 2005; 105(2):621-32
5. El-Sedawy HF, Hussein MMM, Essam T, El-Tayeb OM, Mohammad FHA. â€œScaling Up for the Industrial Production of Rifamycin B Fed-Batch Production Mode in Shake Flasks and Bench-Scale Fermentorâ€, Ferment Technol,2007; 1(5):2167-72
6. Jin ZH, Lin JP, Xu ZN, Cen PL. â€œImprovement of industry-applied rifamycin B-producing strain, Amycolatopsis mediterranei, by rational screeningâ€, J. Gen. Appl. Microbiol,2002; 48(6):329-334.
7. JIN Zhi-hua , LIN Jian-ping, CEN Pei-lin, â€œScale-up of rifamycin B fermentation with Amycolatoposisâ€, Mediterranei Journal of Zhejiang University science,2004; 5(12):1590-96
8. Mandali N, Girijashankar V, Ponamgi SPD, Peela S, Rao LV. â€œRifamycin SV production using immobilized cells of Amycolatopsis mediterranei OVA5-E7 in different matricesâ€, Journal of Chemical and Pharmaceutical Research, 2014; 6(9):298-306
9. Ates S, Elibol M, Mavituna F. â€œProduction of actinorhodin by Streptomyces coelicolor in batch and fedÂbatch culturesâ€, Science Direct, 2010; 32(4):273Â278.
10. Xuan-Tien Doan, Srinivasan R, Bapat PM, Wangikar PP. â€œDetection of phase shifts in batch fermentation via statistical analysis of the online measurements: A case study with rifamycin B fermentationâ€, Journal of Biotechnology,2007; 132(2):156-66
11. Xuan-Tien Doan, Srinivasan R, Bapat PM, Wangikar PP, â€œDetection of phase shifts in batch fermentation via statistical analysis of the online measurements: A case study with rifamycin B fermentationâ€. Current Trends in Biotechnology and Pharmacy, 2010; 3(1):153-61
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