SPECTROSCOPIC DETERMINATION OF TOTAL PHENOL AND FLAVONOID CONTENTS OF CITRUS LIMON PEEL FROM NORTH EASTERN REGION OF INDIA

  • Elija Khatiwora Dept. of Chemistry, Y. M. College, Bharati Vidyapeeth University, Pune 411038, India
  • Vaishali B Adsul Dept. of Chemistry, Y. M. College, Bharati Vidyapeeth University, Pune 411038, India
  • Rasika C Torane Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
  • Sucheta Gaikwad Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
  • Nirmala R Deshpande Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
  • Rajashree V Kashalkar Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India

Abstract

Citrus fruit plants are valuable sources of phenolic and other biologically active compounds.  Phenolic compounds have been reported to have multiple biological effects. In this paper quantitative determination of phenols and flavonoids of citrus limon peel extracts was carried out using spectrophotometric method. The plant material was collected from Assam, north-eastern region of India. Total phenolic content was determined by Folin-Ciocalteau method and aluminium chloride method was used for flavonoid determination. Methanol and ethanol extracts of ripe and unripe peel of the plant contain high amount of phenol contents compared to acetone and ethyl acetate extracts. Methanol extract contains maximum and the ethyl acetate shows minimum amount of flavonoid contents. However, all the extracts show significant results.

Key Words: Citrus limon, Phenol, Flavonoid, Catechol and Quercetin.

 

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Author Biographies

Elija Khatiwora, Dept. of Chemistry, Y. M. College, Bharati Vidyapeeth University, Pune 411038, India
Dept. of Chemistry, Y. M. College, Bharati Vidyapeeth University, Pune 411038, India
Vaishali B Adsul, Dept. of Chemistry, Y. M. College, Bharati Vidyapeeth University, Pune 411038, India

Dept. of Chemistry, Y. M. College, Bharati Vidyapeeth University, Pune 411038, India

Rasika C Torane, Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
Sucheta Gaikwad, Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
Nirmala R Deshpande, Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
Rajashree V Kashalkar, Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India
Dr. T. R. Ingle Research Laboratory, Dept. of Chemistry, Sir Parashurambhau College, Pune 411030, India

References

1. Kahkone MP, Hopia AI, Vourela JH, Rauha JP, Pihlaja K, Kujala TS, Heinonen M, Antioxidant activity of plant extracts containing phenolic compounds, J Agric Food Chem, 1999; 47: 3954-3962.
2. Citrus fruit biology, tehnology and evaluation. Ladaniya: Academic Press; 2008.
3. Mohammadian MA, Mobram ZI, Sajedi RH, Bioactive compounds and antioxidant capacities in the flavedo tissue of two citrus cultivars under low temperature, Braz J Plant Physiol, 2011; 23(3): 203-208.
4. Mukhim C, Nath A, Deka B, Swer TL, The Bioscan, 2015; 10(2): 535-537.
5. Varies PS, Indian medicinal plants. Kottakkal: Arya Vaidya Sala; 2006; 2:97-100.
6. Munne S, Parwate D, Ingle V, Nagpurkar V, Evaluation of the anthelmintic activity of citrus limon juice sacs, Int J Pharma Professional’s Research, 2011; 2(2) .
7. Fejzić A, Ćavar S, Phenolic Compounds and Antioxidant Activity of Some Citruses, Bulletin of the Chemists and Technologists of Bosnia and Herzegovina, 2014; 42:1-4.
8. Barua P, Yield, fruit quality and water productivity of drip fertigated Assam Lemon (Citrus limon). Internat J Agric Engg., 2013; 6(2):339-344.
9. Chaliha BP, Barua AD, Siddappa GS, Assam limon as a source of pactin, Indian Food Packer, 1963, XVII(3).
10. Sarmah N, Kumari S, Comparative study of antibacterial activity of ripen and unripen Indigenous Citrus union of Assam, India, Int J Advancements in Research & Technology, 2013; 2(9):25-31.
11. Deb Roy S, Bania R, Chakraborty J, Goswami R, Laila R, Ahmed SA, Pharmacognostic, phytochemical, physicochemical property and antimicrobial activity studies of lemon peel oil, Nat Prod Plant Resource, 2012; 2(3):431-435.
12. Muthiah PL, Umamaheswari M, Kumar AK, In vitro antioxidant activities of leaves, fruits and peel extracts of citrus, International Journal of Phytopharmacy Research Article, 2012; 2(1):13-20.
13. Malik EP, Singh MB, Plant Enzymology and Hittoenzymology. 1st Ed. New Delhi: Kalyani Publishers; 1980. P. 286.
14. Mervat MM, H El-Far, AA Taie, Antioxidant activities, total anthrocynins, phenolics and flavonoids contents of some sweet potato genotypes under stress of different concentrations of sucrose and sorbitol, Australian J Basic Applied Sc, 2009; 3:3609-3616.
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Khatiwora E, Adsul V, Torane R, Gaikwad S, Deshpande N, Kashalkar R. SPECTROSCOPIC DETERMINATION OF TOTAL PHENOL AND FLAVONOID CONTENTS OF CITRUS LIMON PEEL FROM NORTH EASTERN REGION OF INDIA. JDDT [Internet]. 11Jan.2017 [cited 27Nov.2021];7(1):21-4. Available from: http://jddtonline.info/index.php/jddt/article/view/1368