PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIFUNGAL ACTIVITY OF ESSENTIAL OIL FROM THE RHIZOME OF BERGENIA STRACHEYI (Hook.f. & Thomson) Engl.

  • Pawan Kumar Department of Chemistry, Kumaun University, Nainital 263002, Uttarakhand, India
  • Mohammad Suhail Javed Kumaun University, Nainital 263002, Uttarakhand, India
  • A K Tewari Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar 263145, Uttarakhand, India
  • K S Bisht Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar 263145, Uttarakhand, India

Abstract

The essential oil from the rhizome of Bergenia stracheyi (Hook.f. & Thomson) Engl. growing in the Himalayan region of Jammu & Kashmir is found rich in oxygenated sesquiterpenoids (43.06%) and higher oxygenated hydrocarbons (29.83%). Sesquiterpenoids (0.46%) and monoterpenoids (0.24%) were almost absent in the oil. Among oxygenated sesquiterpenoids Cadinene <14-hydroxy-δ-> (38.36%) and Khusinol (2.06%) were the main components. The higher hydrocarbons and their derivatives include n-Hexadecanoic acid (10.60%), cis-9-hexadecenoic acid (4.46%), Linoleic acid (2.85%), Octacosane (2.52%), Cetanol (2.26%), Oleic acid (2.14%), Nonacosane (1.86%), n-Pentacosane (1.66%) and Untriacontane (1.48%). The oxygenated monoterpenoids include α-Terpineol (1.10%) as main constituent. The oil also contains m-xylene (11.27%) as the major lower hydrocarbon. The decoction of rhizome from Bergenia stracheyi are used in folk lore medicines in Jammu & Kashmir. The oil from the rhizome has shown moderate antifungal activity with 100% mycelia growth inhibition against S. sclerotiorum and A. tenuis at a concentration of 2000µg/ml and 3000µg/ml respectively. However C. graminicola and F. oxysporum were found less susceptible to this oil. The IC50 values showed a range from 802.7µg/ml to 1615.4µg/ml as compared to standard fungicides with IC50 values ranging from 36.8µg/ml - 78.6µg/ml. The spore germination inhibition test has revealed this oil as a moderate inhibitor with IC50 values 899.6µg/ml, 545.2µg/ml and 2014.7µg/ml for A. tenuis, C. graminicola and F. oxysporum spores respectively.

Keywords: Saxifragaceae, Alternaria, essential oil, fungicide, terpenoids.

 

Keywords: Saxifragaceae, Alternaria, essential oil, fungicide, terpenoids

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Author Biographies

Pawan Kumar, Department of Chemistry, Kumaun University, Nainital 263002, Uttarakhand, India

Department of Chemistry, Kumaun University, Nainital 263002, Uttarakhand, India

Mohammad Suhail Javed, Kumaun University, Nainital 263002, Uttarakhand, India

Kumaun University, Nainital 263002, Uttarakhand, India

A K Tewari, Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar 263145, Uttarakhand, India

Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar 263145, Uttarakhand, India

K S Bisht, Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar 263145, Uttarakhand, India

Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture & Technology, Pantnagar 263145, Uttarakhand, India

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Kumar, P., Javed, M., Tewari, A., & Bisht, K. (2017). PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIFUNGAL ACTIVITY OF ESSENTIAL OIL FROM THE RHIZOME OF BERGENIA STRACHEYI (Hook.f. & Thomson) Engl. Journal of Drug Delivery and Therapeutics, 7(1), 37-43. https://doi.org/10.22270/jddt.v7i1.1365