IN-VITRO CYTOTOXICITY ANALYSIS OF TAMOXIFEN CITRATE LOADED CROSS-LINKED GUAR GUM NANOPARTICLES ON JURKAT (HUMAN T-CELL LEUKEMIA) CELL LINE

  • Jayanta K Sarmah Department of Chemistry, Gauhati University
  • Rita Mahanta Cotton College, Guwahati.
  • Saibal Kanti Bhattacharjee Gauhati University.
  • Ranadeep K Mahanta Jorhat Medical College
  • Anindya Dey Dr. B.C. Roy Postgraduate Institute of Basic Medical Sciences and Institute of Post Graduate Medical Education & Research (I.P.G.M.&R.), Kolkata, India
  • Prasun Guha Dr. B.C. Roy Postgraduate Institute of Basic Medical Sciences and Institute of Post Graduate Medical Education & Research (I.P.G.M.&R.), Kolkata, India
  • Angshuman Biswas Assistant Biochemist Central Drugs Laboratory, Kolkata

Abstract

The present investigation was aimed to study the antiproliferative action of tamoxifen citrate (TMX), a non steroidal antiestrogen, on a human T-cell leukemia cell line, Jurkat, as free drug and TMX loaded guar gum nanoparticles. For this we developed a new formulation containing chemically cross-linked guar gum nanoparticles (GG NPs) loaded with tamoxifen citrate (TMX). Single step (oil in water) emulsion and in-situ polymer cross-linking technique was employed to prepare spherical and smooth surfaced nanoparticles in the size range of 200-300nm. Nanoparticle size and shape was confirmed from observation in transmission electron microscope (TEM) analysis. Cytotoxicity on Jurkat (human T-cell leukemia) cell lines as determined by cell growth inhibition after 48 hrs of incubation indicated that tamoxifen citrate loaded guar gum nanoparticles were as efficient as the free drug when applied to the cancer line. However, the crosslinked guar gum nanoparticles loaded with tamoxifen citrate exhibited sustained release of the drug and delayed apoptosis over a long period of time making it suitable for cancer treatment.

Keywords: Nanoparticles, guar gum, tamoxifen citrate, Jurkat (human T-cell leukemia) cell line, cytotoxicity, cell death, WST-1 assay.

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Author Biographies

Jayanta K Sarmah, Department of Chemistry, Gauhati University

Guwahati, Assam.

Pin- 781014

Rita Mahanta, Cotton College, Guwahati.
Associate Professor.
Saibal Kanti Bhattacharjee, Gauhati University.

Prof. of Chemistry

Gauhati University, Guwahati, Assam.

Ranadeep K Mahanta, Jorhat Medical College

Prof. and Head, Dept. of Biochemistry

Jorhat, Assam, India.

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1.
Sarmah J, Mahanta R, Bhattacharjee S, Mahanta R, Dey A, Guha P, Biswas A. IN-VITRO CYTOTOXICITY ANALYSIS OF TAMOXIFEN CITRATE LOADED CROSS-LINKED GUAR GUM NANOPARTICLES ON JURKAT (HUMAN T-CELL LEUKEMIA) CELL LINE. JDDT [Internet]. 13Mar.2012 [cited 24Oct.2020];2(2). Available from: http://jddtonline.info/index.php/jddt/article/view/122