DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF DORZOLAMIDE AND TIMOLOL MALEATE IN PHARMACEUTICAL DOSAGE FORMS
AbstractA fast, sensitive and accurate reverse phase liquid chromatographic method was developed and validated for the simultaneous determination of Dorzolamide and Timolol maleate in ophthalmic preparations. Chromatographic separation was achieved on Inertsil ODS 3V C18 column (250 X 4.6 mm, 5 Âµm particle size) with mobile phase consisting of Acetonitrile and 1-Octane Sulphonic acid buffer (0.02M) pH adjusted to 3.5 Â± 0.05 with o-phosphoric acid (36:64 V/V) at a flow rate of 1.0 mL/min. The analytes were detected at 254 nm and 295 nm for Dorzolamide and Timolol maleate respectively by PDA detector. Brimonidine was used as internal standard (IS). The retention time of Dorzolamide, Timolol maleate and Brimonidine were found to be at 6.020 Â± 0.02, 8.254 Â± 0.01 and 4.636 Â± 0.01 mins respectively. The linearity of the method ranged between 4-720 and 1-180 Âµg/mL for Dorzolamide and Timolol maleate respectively with correlation coefficient 0.999 for both the drugs in binary mixture. The LOD was found to be 0.6951 Âµg/mL and 0.2489 Âµg/mL for Dorzolamide and Timolol maleate respectively and LOQ was found to be 2.3214 Âµg/mL and 0.8317 Âµg/mL for Dorzolamide and Timolol maleate respectively.
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Mathrusri Annapurna M, Narendra A, Deepika D. DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF DORZOLAMIDE AND TIMOLOL MALEATE IN PHARMACEUTICAL DOSAGE FORMS. JDDT [Internet]. 13Mar.2012 [cited 24Oct.2020];2(2). Available from: http://jddtonline.info/index.php/jddt/article/view/120
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