ISOLATION AND EVALUATION OF SEED COAT CONSTITUENTS OF MORINGA OLEIFERA
Abstract
Traditionally, Moringa oleifera seed powder has been used as eco-friendly clarifying agent for drinking water. Natural polysaccharides are widely used as excipients in pharmaceutical industry. In the present In vitro study, starch and protein-mucilage fractions were isolated from the seed coats of Moringa oleifera. The isolated fractions were evaluated as binder and disintegrant in the preparation of paracetamol tablets. The prepared tablets were assessed for comparative In vitro quality control parameters such as weight variation, hardness, friability and disintegration time. The parameters were compared with paracetamol tablets prepared using potato starch as binder and disintegrant. Significant variation was observed in hardness, friability, and disintegration time among three formulations. Paracetamol tablets with protein-mucilage fraction were found to be relatively harder, less friable, and taking more time to disintegrate than the tablets made with potato starch. The tablets with isolated starch fraction were found to be almost similar to tablets prepared with potato starch with respect to hardness, friability and disintegration time. The isolated starch fraction and protein-mucilage fractions exhibited good binding and disintegrating properties and were natural in origin, nontoxic, biodegradable and bio compatible. Hence, they could be employed as binding and disintegrating agents in the formulation of paracetamol immediate release dosage forms. Since the protein fraction showed relatively higher values of hardness and disintegration time with less friability and could be explored for designing sustained release paracetamol tablets.
Key words: Moringa seeds, starch fraction, protein-mucilage fraction, Paracetamol tablets, binder and disintegrant
Â
Downloads
References
2. Bhoomika, R.G., Babita, B.A., Ramesh, K.G and Anita, A.M, Phyto-pharmacology of Moringa oleifera Lam.o an overview, Natural product radiance, 2007, 6(4), pp 347-353.
3. Bhatta charya, S.B., Das, A.K., Banerji, N. Chemical- investigations on the gum exudates from sajna (Moringa oleifera), carbohydr. Res, 1982, 102:253-262.
4. Bennet, R.N., Mellon, F.A., Foidl, N.,Pratt, J.H.,Dupont, M.S.,Perkins, L.,Kroon, P.A Profiling glucosinolates and phenolics in vegetative and reproductive tissues of the multi-purpose trees Moringa oleifera L.and M.stenopetala L,J Agric food chem., 2003,51(12):3546-53.
5. Oleivera, J.T.A., Silveira, S.B., compositional and nutritional attributes of seeds from the multipurpose tree Moringa oleifera Lamarck, J Sci Food Agric, 1999, 79(6): 815-820.
6. Anwar, F., Bhanger, M.I. , Analytical characterization of Moringa oleifera seed oil grown in temperate region of Pakistan, J Agric Food Chem, 2003, 51(22): 6558-6563.
7. Mangale, S. M., Chonde S. G., Jadhav A. S., and Raut P.D, Study of Moringa oleifera (Drumstick) seed as natural Absorbent and Antimicrobial agent for River water treatment. 2012.
8. Fiebich, B.L., Lieb, K., Hull, M., Aicher, B., Ryn, J.V., Pairet, M., Engelhardt, G,Effects of Caffeine and Paracetamol alone or in combination with acetylsalicylic acid on Prostaglandin E2 synthesis in rat microglial cells. Neuropharmacology, 2000, 39(11):2205-2213.
9. Kalakuntla, R., Veerlapati, U., Chepuri, M., Raparla, R, Effect of various super disintegrates on hardness, disintegration and dissolution of drug from dosage form. J. Adv. Sci. Res, 2010, 1(1): 15-19.
10. Santos, A.F.S., Luz, L.A., Argolo, A.C.C., Teixeira, J.A., Paiva, P.M.G., Coelho, L.C.B.B, Isolation of seed coagulant Moringa oleifera lectin, Process biochemistry, 2009, 44:504-508.
11. Khandelwal KR, In: Practical Pharmacognosy, Techniques and experiments, 2nd edition, Nirali Prakashan, 2000, pp.149-156.
12. Vasudevan TN and Laddha KS, In : Practical Pharmacognosy, 1st Edition, New Vrinda Publishing house, MG Road, Jalgaon, 1992, pp.77.
13. Banker, G.S., Anderson, N.R,Tablets in Lachman. L. and Lieberman, H.A, The theory and practice of industrial pharmacy, Special Indian ed., 2009, pp 229-345, CBS Publishers and Distributors Pvt. Ltd., India.
14. US Pharmacopeia National Formulary USP 23/NF 18, United States Pharmacopeial Convention. Inc., Rockville,MD, 1995.
15. Marques, M.R.F., and Barracco, M.A. (2000): Lectins, as non-self recognition factors, in crustaceans. Aquaculture, 191: 23-44.
16. Bies, C., Lehr, C.M., and Woodley, J.F., Lectin mediated drug targeting: History and applications, Advanced Drug Delivery Review, 2004, 56: 425-435.
17. Nachbar and Oppenheim., Lectins in the United States Diet. In: American Journal of Clinical Nutrition, 1980, 33:2338-2345.
18. Ngwuluka, N. C., Idahoan, B. A. NEP, E. I., Ogaji. I., Okafor, I. S., Formulation and evaluation of paracetamol tablets manufactured using the dried fruit of Phoenix dactylifera Linn as an excipient, 2010.
19. Kalu, V.D, and Odeniyi, M.A., Jaiyeoba KT. Matrix properties of a new plant gum in controlled drug delivery. Arch Pharm Res, 2007, 30:884-9.
20. Ogaji, I., Nnoli, O., Film coating potential of okra gum using paracetamol tablets as a model drug. Asian J Pharma, 2010, 4:130-4.
21. Attama, A.A., Adikwu, M.U., and Amorha, C.J, Release of indomethacin from bioadhesive tablets containing Carbopol® 941 modified with Abelmuschus esculentus (Okra) gum. Boll Chim Farm, 2003, 142:298.
22. Tousey, M.D,ablet pro: A tablet making training resource for tablet making professionals Techceuticls, 2011, 4(1):1-15. www.dipharma.comTP_V4.pdf [Accesses on: 09.03.2012].
23. Musa, H., Sule, Y.Z., and Gwarzo, M.S,Assessment of physicochemical properties of metronidazole tablets marketed in Zaria, Nigeria. Int J Pharmacy Pharm Sci, 2011, 3(Suppl 3): 27-29.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).