DEVELOPMENT OF MUCOADHESIVE MICROSPHERES OF LEVOFLOXACIN FOR THE TREATMENT OF H. PYLORI INFECTION

  • Keerthi Sumana Murathoti Gokaraju Rangaraju college of Pharmacy, Osmania University
  • Trapti Saxena Dr. Harisingh Gour Vishwavidhyalaya

Abstract

The rationale of the present investigation is to develop a new oral drug delivery system utilizing both the concepts of controlled release and mucoadhesiveness, which could remain in stomach and control the drug release for longer period of time and thus to improve the bioavailability of the drug and reducing its dose related side effects. Gelatin/Acrypol 934P mucoadhesive microspheres of Levofloxacin were prepared by Emulsification cross- linking method. Drug and excipients interactions are tested using Fourier Transform-Infrared Spectroscopy, indicated no interactions. The average particle size for optimized formulations of Gelatin/Acrypol 934P microspheres were found to be 41.5µm. Photomicrographs revealed that the microspheres were spherical in shape. The drug entrapment efficiency for Gelatin/ Acrypol 934P microspheres was found to be 85.43%. In vitro drug release from Gelatin/ Acrypol microspheres showed more than 75% of the drug was released within 8 Hr, while pure drug showed complete drug release within 3 hours. This suggested controlled delivery of Levofloxacin for a longer period. Regression analysis revealed that the drug release from the microspheres was followed zero order kinetics. SEM images suggested spherical shape with smooth surface of microspheres formulations. Optimized formulation of Gelatin/ Acrypol 934P microspheres showed excellent mucoadhesivity i.e., 86.5%. Thus, the proposed Gelatin/ Acrypol 934P mucoadhesive microspheres might make a contribution in complete eradication of Helicobacter pylori owing to prolonged stomach residence time and small particle size.

Keywords: Levofloxacin, H. pylori, Gelatin, Acrypol 934P, mucoadhesion, controlled release.

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Author Biographies

Keerthi Sumana Murathoti, Gokaraju Rangaraju college of Pharmacy, Osmania University
Industrial Pharmacy
Trapti Saxena, Dr. Harisingh Gour Vishwavidhyalaya
Pharmaceutical science

References

1. Martin J. B. The Bacteria behind Ulcers. Issue of the Scientific American. February 1996; pg. 104-107.
2. Akamatsu T et al. Transmission of Helicobacter pylori infection via flexible fiberoptic endoscopy. American Journal of Infection Control. 1996; 24: 396–401.
3. Park K., Robinson J. R., Bioadhesive polymers platforms for oral controlled drug delivery. Int. J. Pharma. 1984; 19: 107- 127.
4. Chien YW. Concepts and System Design for Rate-controlled Drug Delivery, Chapter 1 in Novel Drug Delivery System. 2nd Edition Marcel Dekker, Inc, New York, 1992; l-42.
5. Chowdary KPR, Srinivasa Rao Y. Mucoadhesion properties in relation to microspheres. American Association of Pharmaceutical Scientists Technology. 2003; 4: 320-325.
6. Desai V. N. , Ozadheoghene E. A. , Dagunduro B. O. , Okonkwo T.J. A Simple UV Spectrophotometric Method for the Determination of Levofloxacin in Dosage Formulations. Tropical Journal of Pharmaceutical Research. February 2011; 10 (1): 75-79.
7. Hitesh B. G., Suresh G., Nitin P. Formulation of characterisation of Levofloxacin loaded biodegradable nanoparticles. Asain Journal of Pharmaceutics. 2011; vol. 2: 114- 119.
8. Leucuta S. E., Ponchel G. and Duchene D. Dynamic swelling behaviour of gelatin/ poly (acrylic acid) bioadhesive microspheres loaded with oxprenolol. National Institute of Health. 1997; Vol. 14: No. 4 , Pages 501-510.
9. Brahmaiah B, Prasanna Kumar. Formulation and evaluation of extended release mucoadhesive microspheres of simvastatin. International Journal of Pharmaceutical and Biomedical research. 2013; 4(1), 57- 64.
10. Harshad parmar et al. Different Methods of Formulation and Evaluation of Mucoadhesive Microspheres. International Journal Applied Biology Pharmaceutical Technology. 2010; 1 (3): 1157-1167.
11. Jayvadan P. and Jayant R. Chavda. Formulation and evaluation of stomach- specific amoxicillin- loaded carbopol- 934P mucoadhesive microspheres for anti- Heliobacter pylori therapy. Journal of Microencapsulation. 2009; 26 (4): 365- 376.
12. Shahnooshi Javad F., Anita Dadollahi Sarab, Jain Akshat, Design and Evaluation of Mucoadhesive Microspheres of Nateglinide. American journal of pharmatech research. 2013; 3(6), 2249-3387.
13. Brahmaiah B, Prasanna Kumar. Formulation and evaluation of extended release mucoadhesive microspheres of simvastatin. International Journal of Pharmaceutical and Biomedical research. 2013; 4(1), 57- 64.
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Murathoti KS, Saxena T. DEVELOPMENT OF MUCOADHESIVE MICROSPHERES OF LEVOFLOXACIN FOR THE TREATMENT OF H. PYLORI INFECTION. JDDT [Internet]. 15Jan.2016 [cited 27Feb.2020];6(1):34-5. Available from: http://jddtonline.info/index.php/jddt/article/view/1179