LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS QUANTITATIVE DETERMINATION OF CANDESARTAN CILEXETIL AND HYDROCHLORTHIAZIDE IN PHARMACEUTICAL DOSAGE FORMS
A simple and sensitive RP-HPLC method was developed and validated for the determination of Candesartan cilexetil and Hydrochlorthiazide in pharmaceutical dosage forms. The separation of components was achieved on a SHIMADZU Hypersil ODS-C18 column (250 Ã— 4.6 mm, 5 Âµm) with UV detection at 270 nm. Isocratic elution with a mobile phase consisting of 10 mM (pH 3.37) Tetra butyl ammonium hydrogen sulphate: methanol (15:85, V/V), at a flow rate 1.0 mL min-1 was employed. Linearity was observed in the concentration range 0.625-62.5 Âµg/mL for Hydrochlorthiazide and 0.8-80 Âµg/mLfor Candesartan cilexetil respectively. The linear regression equation was found to be Y=64002X-1412.6 for Hydrochlorthiazide and Y=24649X-6701.8for Candesartan cilexetil respectively with correlation coefficients greater than 0.999. The LOD was found to be 0.1385 and 0.1892 Âµg/mL for Hydrochlorthiazide and Candesartan cilexetil respectively where as the LOQ was found to be 0.4394 and 0.6187 Âµg/mL for Hydrochlorthiazide and Candesartan cilexetil respectively. The mean analytical recovery in determination of Candesartan cilexetil and Hydrochlorthiazide tablets was 99.31-100.08% Hydrochlorthiazide and 99.58-100.39% for Candesartan cilexetil respectively. Thus, the proposed method is applicable for routine determination of Candesartan cilexetil and Hydrochlorthiazide in pharmaceutical formulations.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).