DEVELOPMENT AND VALIDATION OF REVERSED PHASE HPLC METHOD FOR ESTIMATION OF CEFTRIAXONE IN PHARMACEUTICAL DOSAGE FORM
A simple, accurate rapid and precise RP-HPLC method has been developed and validated for determination of Ceftriaxone in bulk drug. The RP-HPLC separation was achieved on Promosil C-18, (250 mm, 4.6 mm, 5Âµm) using mobile phase buffer: methanol ph 6.8 (90: 10 v/v) at flow rate of 1.0 ml/min at ambient temperature. The retention times were 7.111 min. for Ceftriaxone. Calibration plots were linear over the concentration range 1-20Âµg/ml. Quantification was achieved with photodiode array detection at 260 nm over the concentration range of 1-50 Âµg/ml. The method was validated statistically and applied successfully for the determination of Ceftriaxone. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for the routine determination of Ceftriaxone in bulk drug.
KeywordsÂ Â Â Ceftriaxone, Water, Buffer, Validation, HPLC.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).