EXPLORATION OF ANTI-HYPERGLYCEMIC AND HYPOLIPIDEMIC ACTIVITIES OF ETHANOLIC EXTRACT OF TINOSPORA CARDIFOLIA (WILD)HOOK WHOLE PLANT IN ALLOXAN INDUCED DIABETIC RATS

  • T Naga Ravikiran Research scholar, Dept. of Pharmaceutical chemistry, A U college of Pharmaceutical Sciences, Andhra University,Visakhapatnam.
  • AVS Madhulatha Dept of pharmaceutics,Krishna university
  • D Lohithasu Dept of pharmaceutics,Gitam University
  • Y Rajendra Prasad Research scholar, Dept. of Pharmaceutical chemistry, A U college of Pharmaceutical Sciences, Andhra University,Visakhapatnam.

Abstract

Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for diabetes mellitus. The aim of present study was to demonstrate the hypoglycemic and anti-diabetic activity of the Ethanolic extract of tinospora cardifolia whole plant in alloxan induced diabetic animals with a view to explore its use for the treatment of diabetes mellitus in humans. The Ethanolic extract of tinospora cardifolia whole plant was investigated for its anti-hyperglycemic and anti-hyperlipidemic effects in male albino rats. Diabetes was induced in the albino rats by administration of a single dose of alloxan monohydrate (150 mg/kg, bwt, i.p) and the Ethanolic extract of tinospora cardifolia whole plant was administered daily at single doses of 100 and 200 mg/kg, p. o to diabetes induced rats for a period of 14 days. The effect of Ethanolic extract of tinospora cardifolia whole plant on blood glucose level was measured in the diabetic rats. Serum lipid profiles [total cholesterol, triglycerides, phospholipids (low density, very low density and high density lipoprotein)] were also determined. The activities were also compared to the activity produced by a standard anti diabetic agent, Glibenclamide (500 μg/kg). The present investigation established pharmacological evidence to support the folklore claim that Ethanolic extract of tinospora cardifolia whole plant is an anti-diabetic agent.

Keywords : Diabetes, Tinospora cardifolia,Alloxan, HDL,VLDL and fasting blood glucose

Keywords: Diabetes, Tinospora cardifolia, Alloxan, HDL, VLDL and fasting blood glucose

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Author Biographies

T Naga Ravikiran, Research scholar, Dept. of Pharmaceutical chemistry, A U college of Pharmaceutical Sciences, Andhra University,Visakhapatnam.

Research scholar, Dept. of Pharmaceutical chemistry, A U college of Pharmaceutical Sciences, Andhra University,Visakhapatnam.

AVS Madhulatha, Dept of pharmaceutics,Krishna university

Dept of pharmaceutics,Krishna university

D Lohithasu, Dept of pharmaceutics,Gitam University

Dept of pharmaceutics,Gitam University

Y Rajendra Prasad, Research scholar, Dept. of Pharmaceutical chemistry, A U college of Pharmaceutical Sciences, Andhra University,Visakhapatnam.

Research scholar, Dept. of Pharmaceutical chemistry, A U college of Pharmaceutical Sciences, Andhra University,Visakhapatnam.

References

1).El-Soud NHA, Khalil MY, Hussein JS, Oraby FSH, Farrag FAR. Anti-diabetic effects of fenugreek alkaliod extract in streptozotocin induced hyperglycemic rats. J Appl Sci Res, 3, 2007, 1073-1083.

2). D. O. Adeyemi, O. A. Komolafe, S. O. Adewole, E. M. Obuotor: Anti Hyperlipidemic Activities of Annona Muricata (Linn). The Internet Journal of Alternative Medicine. 7(1), 2009, DOI: 10. 5580/293b.

3) Rajesh Kumar Gupta, Achyut Narayan Kesari, Geeta Watal, P. S. Murthy, Ramesh Chandra, Kapil Maithal and Vibha Tandon, Hypoglycaemic and antidiabetic effect of aqueous extract of leaves of Annona squamosa (L.) in experimental animal, CURRENT SCIENCE, 88, 25 APRIL 2005, 1244.

4) Anonymous. Wealth of India: Raw materials. CSIR, New Delhi, 1976, 10.

5). Chopra RN, Chopra LC, Handa KD, Kapur LD, editors. Indigenous Drugs of India. 2nd ed. Kolkata: M/S Dhar VN & Sons; 1982.

6). Zhao TF, Wang X, Rimando AM, Che C. Folkloric medicinal plants: Tinospora sagittata var. cravaniana and Mahonia bealei. Planta Med 1991;57:505.

7).Nayampalli S, Ainapure SS, Nadkarni PM. Study of antiallergic acid Bronchodilator effects of Tinospora cordifolia. Indian J Pharm 1982;14:64-6.

8). Agarwal SK, Singh SS, Verma S, Kumar S. Two picrotoxin derivatives from Anamirta cocculus. Phytochemistry 1999;50:1365-8.

9). Agarwal SK, Singh SS, Verma S. Antifungal principle of sesquiterpene lactones from Anamirta cocculus. Indian Drugs 1999;36:754-5.

10). Khosa RL, Prasad S. Pharmacognostical studies on Guduchi (Tinospora cordifolia Miers). J Res Ind Med 1971; 6:261-9.

11). Mehra PN, Puri HS. Studies on Gaduchi satwa. Indian J Pharm 1969;31:180-2.

12). Rao EV, Rao MV. Studies on the polysaccharide preparation (Guduchi satwa) derived from Tinospora cordifolia. Indian J Pharm Sci 1981;43:103-6.

13). Chintalwar G, Jain A, Sipahimalani A, Banerji A, Sumariwalla P, Ramakrishnan R, et al. An immunologically active arabinogalactan from Tinospora cordifolia. Phytochemistry
1999;52:1089-94.

14). P. H. Mueller, R. M. Schmuelling, and H. M. Liebich, “A fully enzymatic triglyceride determination,” Journal of Clinical Chemistry and Clinical Biochemistry, 15, 1977, 457–464.

15) C. C. Allain, L. S. Poon, and C. S. G. Chan, “Enzymatic determination of total serum cholesterol,” Clinical Chemistry, 20, 1974, 470–475.

16) W. T. Friedewald, R. I. Levy, and D. S. Fredrickson, “Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge,” Clinical Chemistry, 18, 1972, 499–502.

17). D. O. Adeyemi, O. A. Komolafe, S. O. Adewole, E. M. Obuotor: Anti Hyperlipidemic Activities of Annona Muricata (Linn). The Internetional Journal of Alternative Medicine. 7, 2009, DOI: 10. 5580 / 293b.

18). D. O. Adeyemi1, O. A. Komolafe1, O. S. Adewole1, E. M. Obuotor2, A. A. Abiodun1, T. K. Adenowo3, Histomorphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extracts of Annona muricata. Folia Morphol. 69, No. 2, 92–100.

19).Urmila C. Kumavat, Shraddha N. Shimpi,1 and Sandesh P. Jagdale, Hypoglycemic activity of Cassia javanica Linn. In normal and streptozotocin-induced diabetic rats Journal of Advanced pharmaceutical Technology and Research, 3(1), 2012, 47–51.

20). Lehto S, Haffner SM, Pyörälä K, Kallio V, Laakso M. Dyslipidemia and hyperglycemia predict coronary heart disease events in middle-aged patients with NIDDM; Diabetes 1997; 46, 1354–1359.

21). Eisenberg DM, Kessler RC, Foster C, Norlock FE, Calkins DR,Delbanco TL Unconventional medicine in theUnited States. Prevalence, costs, and patterns of use. N Engl J Med, 328, 1993, 246–252.

22). Chattopadhyay RR A comparative evaluation of some blood sugar lowering agents of plant origin.J Ethnopharmacol, 67, 1999, 367–372.

23). Kim JD, Kang SM, Park MY, Jung TY, Choi HY, Ku SK, Ameliorative antidiabetic activity of Dangnyosoko, a Chinese herbal medicine in diabetic rats. Biosci Biotechnol Biochem, 71, 2007, 1527–1534.

24) S.S. Singh, S.C. Pandey, S. Srivastava, V.S. Gupta, B. Patro, A.C. Ghosh Chemistry And Medicinal Properties Of Tinospora Cordifolia (Guduchi) Indian Journal Of Pharmacology 2003; 35: 83-91.
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Ravikiran T, Madhulatha A, Lohithasu D, Prasad Y. EXPLORATION OF ANTI-HYPERGLYCEMIC AND HYPOLIPIDEMIC ACTIVITIES OF ETHANOLIC EXTRACT OF TINOSPORA CARDIFOLIA (WILD)HOOK WHOLE PLANT IN ALLOXAN INDUCED DIABETIC RATS. JDDT [Internet]. 14Mar.2015 [cited 18Feb.2020];5(2):94-9. Available from: http://jddtonline.info/index.php/jddt/article/view/1094